box in a class 2 promoter. He got a piece of DNA that had a good class 2 promoter and a piece of DNA that he knew he could cut with restriction endonucleases. The idea is to pair a piece of DNA with label on only one strand. In this case you want the label on the crick strand. You would take this piece of DNA with a hot phosphate on 5 prime end of the crick strand and denature it. The probe DNA helps you see where txn starts. You take that probe DNA and you mix it with transcript thats made by the
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Four bases of segmentation Geographic Collecting and analysing information according to the location of the customer or other data source. Geographic segmentation is often used in marketing‚ since companies selling products and services would like to know where their products are being sold in order to increase advertising and sales efforts there. A local shop such as a local takeaway use geographic segmentation by targeting customers within a 10 mile radius‚ they can do this by posting leaflets
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Name: Chrelle Branche * Class: 404 * Teacher: MissChatoor * Subject: Biology * Due Date: Oct. 5th 2012 * School: Marabella North Secondary * Feeding Relationships Found in Nature. Plants make their own food. Therefore‚ plants are called producers. You know that animals depend on plants or on other animals for their Food. Therefore‚ animals are called consumers. A study of feeding relationships‚ or food chains‚ helps us to better understand our environment. And if we
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Outline the key strands of the sociology of work! The term work is expressed as performing duties involving the use of psychological and physical energy‚ for the purpose of the manufacture of goods and services in order to achieve an individuals needs (Gedden’s 2008). There are six key areas of thought in the sociology of work these include “managerial psychologist”‚ Durkheim systems”‚ Interactionist”‚ Weber Interpretivist”‚ Marxian and “Post structuralise and Post Modern” (Watson 2008). Sociology
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true nature of obsession manipulates a person’s character‚ forcing them to act in unusual ways‚ as if possessed by an involuntary need. An obsessed mind becomes intensely absorbed in the subject of the obsession to an extent that drives away the truth and realism in their values‚ morals and perceptions. William Shakespeare manifests this idea of an involuntary change in character in the play “Othello”‚ and with a similar approach this idea is identified by Scott Spencer in the novel “Endless Love”
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2.1 1. Nature of Individual differences found in organizations The recognition of individual differences is central to any discussion of organizational behavior. Individual differences are the variations from one person to another on variables such as self-esteem‚ rate of cognitive development or degree of agreeableness. Historically‚ psychological science has overlooked individual differences in favor of focusing on average behavior. Individual differences have been most often studied in the area
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So the poet I decided to do this on is Mark Strand. He was born in Canada in 1934 and grew up in different places in the United States. He studied at several universities including Antioch Ohio college‚ Yale‚ also the University of Florence on a Fulbright fellowship where he studied 19th century Italian poetry. When he was a child‚ he actually wasn’t considered bright. He was a painter while studying at Yale‚ and a lot of people say that he creates a “painterly” image in his poetry because
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Raven came up with five different bases of power. Coercive‚ legitimate‚ reward‚ referent‚ and expert are the five types of power. Someone can create power through one or more of these bases. There is the possibility that one base power may undermine another. One must learn how to use these powers in a positive way to create a positive relationship. Coercive power gives employers power by having the employees fearful of punishment. Termination‚ demotion‚ or loss of rewards could be used as punishments
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not enough‚ we must apply. Willing is not enough‚ we must do.” (Johann von Goethe) “The Endless Steppe” by Esther Hautzig is a novel about a family’s determination to survive. In the novel it tells of how determination‚ the human ability to adopt‚ and happiness can hold a family together and help them even preserver over all odds. In addition‚ “The Endless Steppe” tells of Reisa‚ Ryia‚ and Esther tales of how they overcame these ordeals and survived in Siberia in their own way. The theme of this
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DNA DNA‚ or Deoxyribonucleic Acid‚ is described‚ in Encarta Encyclopedia as a genetic material of all cellular organisms and most viruses. DNA carries the information needed to direct protein synthesis and replication. Protein synthesis is the production of the proteins needed by the cell or virus for its activities and development. Replication is the process by which DNA copies itself for each descendant cell or virus‚ passing on the information needed for protein synthesis. In most cellular
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