Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
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pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities‚ one of the enzyme that was used in the process was amylase. When a serial dilution was
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correlation between catalase concentration and the rate of reaction. This occurs because as the enzyme concentration increases‚ there are more enzymes available to catalyze substrates. More enzymes means more reactions can take place at a time‚ thus a faster rate of reaction. Overall‚ based on the results of table 2.0 and graph 2.0‚ it is prevalent that there is a positive correlation between the concentration of enzymes and the rate of
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Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because we assume the more substrate an enzyme gets
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DISCUSSION: Bromelian added to Gelatin: Bromelian is an enzyme found in pineapples. When Bromelian is added to gelatin it breaks down the protein and does not allow the gelatin to solidify. There are several factors that can cause an enzyme to slow down or to completely stop reacting. For example‚ temperature and pH can effect enzyme activity. Canned pineapple juice and fresh pineapple juice were used to see how the enzyme would react differently. In fresh pineapple juice the Bromelian have would
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Enzyme Kinetics Examples and Problems 1. An enzyme is produced for producing a sun protection lotion. Given kinetic data for the enzyme reaction with Vm=2.5 mmol/m3.s‚ Km=8.9 mM and So=12mM‚ what would be the time required for 95% conversion in a batch reactor? 2. An enzyme was assayed at an initial substrate concentration of 10-5M. The Km’ for the substrate is 2x10-3M. At the end of 1 min‚ 2% of the substrate had been converted to product. a. What % of the substrate will be converted
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Conclusion…………………………………………………………………………....…….8 Works Cited ………………………………………………………………………………..9 Properties of Enzymes and Competitive Inhibitors. Abstract: Properties of enzymes were found in this experiment and some other factors‚ which affect enzyme activity. Enzymes are catalyst; they catalyze very specific reactions. Results relating to the active site of specific enzymes played a big role while performing this experiment. The purpose of this experiment was to fin how inhibitors affect
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The verse narrative entitled Gilgamesh written by Herbert Mason presents the theme of self discovery. Gilgamesh is the unrighteous demigod ruler of Uruk. His moral compass can be questioned as he gives his people intensive labour and will sexually harass the women. Consequently‚ he becomes unaware of his limitations because he has absolute power. Inevitably‚ this changes when he meets his near equal Enkidu. They embark on a journey into the forest which holds Humbaba. Enkidu’s fear of death intensifies
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gives the awareness not rely on the materialistic patriarchal society. In the journey of sel- discovery‚ the narrator is collapsed with the memories of the past and her thoughts of her mother give her power and strength about the society and natural world. The narrator considers both herself and her boyfriend Joe as animals. She asks Joe to have physical contact with her. Moreover‚ she forces him for the same at the outside world. Through this action‚ she wants to mingle with this new unborn child
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Simple Experiments on the Enzyme Catalase Aim: The aim of this practical is to use three different techniques to investigate the effect of different concentrations of the enzyme catalase on the rate of breakdown of hydrogen peroxide. Background information Catalase is an enzyme which is found in all living organisms. This enzyme catalases the decomposition of hydrogen peroxide into water and oxygen. Cells continually produce a poisonous by-product of metabolising‚ called hydrogen peroxide. This
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