2.5 Detectors The detectors used in UPLC should be able to handle very fast scanning methods because half-height peak widths of less than one second are usually obtained with columns packed with 1.7 µm particles. The detector must be able to give high sampling rate adequate to capture enough data points across the peak for an accurate and reproducible integration of analyte peak. The dispersion (volume) of the detector flow cell must be minimal to maintain separation efficiency. Conceptually‚ the
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Division of Environmental Epidemiology University Utrecht 05/06/2007 Biopharmaceutical Technology Europe Volume 21‚ Issue 10 Valencio Salema‚ Lalit Saxena‚ Priyabrata Pattnaik ‘Removing endotoxin from biopharmaceutical solutions’ 01/09/2009 ‘Ion Chromatography: A New Technique for Clinical Chemistry’ Courtney Anderson Vol. 22‚ No. 9 1976 Biochemistry Jeremy M. Berg‚ John L. Tymoczko‚ Lubert Stryer 2006 ‘Curtin’s industrial biotechnology research’ (http://www.theborneopost.com/2013/02/12/curtins-industrial-biotechnology-research/)
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filtration is a non-adsorptive chromatography technique that separates molecules on the basis of molecular size. Desalting and buffer exchange are two special examples of gel filtration that are widely used in many downstream bioprocesses. Desalting is used to completely remove or lower the concentration of salt or other low molecular weight components in the sample while buffer exchange replaces the sample buffer with a new buffer. Gel filtration is one of the easiest chromatography methods to perform because
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Gel filtration GEL FILTRATION OF PROTIENS Aim: The aim of this experiment is to identify proteins from a complex mixture using the gel filtration technique also known as size exclusion chromatography. This technique is widely used by biochemists when proteins larger than the pores are excluded from the column and the smaller molecules elute last and then collected in test tubes for examination by spectroscopic techniques. The red/brown proteins‚ in particular‚ will be observed closely
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natural source of vitamin C. Tissue cultures of A. vasica‚ initiated on Murashige and Skoog’s medium supplemented with various plant growth regulators‚ showed the presence of alkaloids‚ vasicine and vasicinone. Water extracts of shoot cultures contained high levels of these alkaloids. The vasicine and vasicinone contents in these extracts were 5.98% and 5.2% of dry weight and the water extracts of the selected elite parent plant contained 3–4% dry weight of vasicine. The methanolic extracts of the parent
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Title of experiment 3: Gel Filtration Chromatography of LDH INTRODUCTION Gel filtration chromatography is a type of column chromatography in which separated protein‚ peptides and amino acids on their molecular size. The stationary phase consists of beads containing pores. The mobile phase is the solvent that is found both around the beads and in the pores of the stationary phase matrix. As the sample is passes through the column‚ the molecule that are larger than the pores will not retarded by
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due to the structure of disperse red 9 being more symmetrical than that of disperse blue 3 and having more nonpolar bonds. Disperse blue 3 is more polar because it has a hydroxide bond and has a larger dipole. The principle behind using column chromatography is that it separates compounds based on polarity. The alumina serves to allow for a purer separation than TLC plates because it has a more polar surface than silica gel does. The less polar dye moves first because it is not as soluble in the stationary
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Experiment # 4 Paper Chromatography 1. Why is the chromatogram developed in an essentially closed system? - The chromatogram is developed in a closed system in order to prevent the solvent to evaporate. Most solvents used in the chromatograph are toxic and flammable. It is also put in a close system to reduce the chance of outside factors affect the chromatograph. 2. What is the main advantage of 2-dimensional paper chromatography over a 1-dimensional one? - The
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A simple‚ specific‚ accurate and rapid reversed phase high performance liquid chromatographic method was developed for the simultaneous estimation of cilnidipine and chlorthalidone‚ using a Hiber® C18 (25cm x 4.6 mm)‚ Lichrospher® 100‚ RP-18 e (5µm) column and a mobile phase composed of phosphate buffer(pH 4.0 adjusted with orthophosphoric acid) : Acetonitrile (30:70‚ v/v). The retention times of Cilnidipine and Chlorthalidone were found to be 3.967 min and 6.427 min‚ respectively. Linearity was
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PROJECT PROPOSAL PROJECT TITLE Isolation of antibacterial protein from coelomic fluid of earthworm-Perionyx excavatus ( Indian blues) and elucidation of its cytotoxic effects PROJECT PROPOSAL 3. PROJECT TITLE: “Isolation of antibacterial protein from coelomic fluid of earthworm - Perionyx excavatus ( Indian blues) and elucidation of its cytotoxic effects” 4. SPECIFIC AREA OF THE PROJECT: “ VERMITECHNOLOGY”
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