ETME 3252 ‚ Fall 2004 Thermodynamics and Heat Transfer Laboratory Manual – 11th edition Edited by: Professor Ed Braun Department of Engineering Technology Copyright ©2004. Material in this document is for your educational use only. This document contains copyrighted and other proprietary information. You may not in any way make commercial or other unauthorized use‚ by publication‚ re-transmission‚ distribution‚ caching‚ or otherwise‚ of this material‚ except as permitted by the Copyright
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Genetic transformation of Escherichia coli with pGLO (Adapted from: Biotechnology Explorer: Bacterial Transformation: The pGLO System. Instructors Guide. BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms.
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Copper (II) Sulfate Purpose: The purpose of this lab is to find out how many moles of water there are in one mole of Copper Sulfate (CuSO x HO) and the percent by mass of water in the hydrate. This will be accomplished by heating a determined mass of the hydrate to remove the water‚ and by measuring the salt left over. Introduction: This laboratory experiment will be done to find out how many moles of HO are present per one mole in the hydrate CuSO. A hydrate is a compound that has a fixed number
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Lab Report Background Information: Magnesium is an alkaline earth metal that has the symbol Mg. Magnesium is a fairly strong‚ silvery-white‚ light-weight metal (one third lighter than aluminum). In a powder‚ it heats and ignites when exposed to moisture and burns with a white flame that is harmful to the eyes. It is difficult to ignite in bulk‚ but once ignited‚ it is difficult to extinguish. Magnesium Ribbon is a long strip of magnesium metal about 3 millimeters wide and 10 meters long with
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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“Fermentation is any process where microorganisms use an external food source for energy. This process is done in a fermenter‚ conditions are controlled by mixing‚ water jacket.” – URL: http://scienceaid.co.uk/biology/micro/fermentation.html Below is the Lab Report of the group for yogurt making activity. II. Materials and Methods * 1 liter homegenous pasteurized milk * 30 grams skim milk powder (SMP) * 100 grams refined sugar * 80 ml starter culture bacteria from natural yoghurt prepared
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Abstract: For the first part of this lab we refluxed different Carboxylic acids and alcohols in the presence of a acid catalyst in order to form Esters by Fischer Esterification. These Esters had different pleasant smells that we then evaluated. In the second part of the experiment‚ we broke the ester bonds of a triglyceride in order to form glycerol and carboxylate salts. This process is known as Saponification because it produces amphiphilic molecules that allow soap to remove dirt from the surface
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Abstract Through three different experiments we were to find the quality of NaCl as a deicer. These experiments included testing the freezing point depression of NaCl in solution at two different concentrations‚ testing for the H of an aqueous NaCl solution‚ and combining NaCl with MgCl2 in an aqueous solution to test for freezing point depression. Through these various experiments we found NaCl to be the best deicer for our region due to its proficient freezing point depression and its cost
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Spencer Richardson Biology 111 Lab 7 Tube Initial Gas Height(mm) Final Gas Height(mm) Net Change 1 .5 .7 + .2 2 .5 .2 -.3 3 .5 .7 + .5 4 .5 1.0 +.5 5 .5 .5 0 1. The clinical formula of Equal was not metabolized because it has no sugar. Splenda is actually derived from sugar so the results would be different. 2. Carbon Dioxide was the gas that was produced. Two methods would be by examining the bottom of the tube and to measure the net difference. 3. The
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In the short story‚ “August Heat‚” W. F. Harvey uses imagery‚ point of view‚ and structure to build intensity‚ and to show how humans cannot control nature. The first person point of view shows how James’s attitude changes over the course of the story. At the beginning‚ James states‚ “I am forty years old‚ in perfect health‚ never having known a day’s illness.” Later in the story‚ James had a‚ “sudden impulse‚” to enter the monumental maker yard. James just deciding to enter the yard brings attention
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