Introduction: Antimicrobial agents are chemicals that are used against bacteria. There are many such agents available. Because there are many different situations where bacterial control is important‚ no antimicrobial agent is effective in all situations. For example‚ you wouldn’t use the same compound to fight an ear infection as you would use to sterilize surfaces in an Operating room. The situations are completely different. In one case‚ you are trying to assist the body to fight off an internal
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CORE BIOLOGY PRACTICALS You will need to know these practicals as the exam board may ask you questions based on them. Below is a summary of each one. Name of practical and independent & dependent variables Effect of caffeine on Daphnia heart rate Independent: caffeine concentration Dependent: heart rate of Daphnia Measuring the content of Vitamin C in fruit juice Independent: fruit juice Dependent: volume of juice required to decolourise 1cm3 of DCPIP The effect of temperature on cell membranes
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The first test I did was the catalase test. It had a positive reaction meaning the bacteria produced the enzyme catalase. This helped me narrow my unknown down to Micrococcus and Staphylococcus. I inoculated my unknown onto a mannitol salt agar. The growth and color change to yellow indicated mannitol fermentation because acid was produced. The acid fermentation is a characteristic of the pathogen
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Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab‚ the Green Fluorescent Protein‚ which is typically found in the bioluminescent jellyfish Aequorea
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Introduction In my report I will discuss what I did as an experiment and what I hope for it to achieve. Firstly I carried out an experiment to assay the effectiveness that a range of disinfectants have on the growth of ecoli and whether or not it can prevent it from growing. From the experiment i should be able to see that some disinfectants have a greater effect than others do. From this I shall then draw a conclusion and evaluation on what was the most effective‚ and could there have been any
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their products. Very few of the things we eat or drink are bacteria free. They merely have greatly reduced numbers of “harmless” bacteria. It is often necessary to determine how many live bacteria are actually in a sample‚ especially when measuring growth rates or determining disinfectant effectiveness. This involves MacConkey agar which is a selective and differential
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The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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Lab: Sampling Bacteria Purpose: Refer to handout sheet. Materials: Refer to handout sheet. Procedure: Refer to handout sheet. Pre-Lab Questions: 1. Why is one dish being reserved for the class as a "control"? Having a controlled variable is important in order to be able to look at what the bacteria would look like if it hadn’t been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar
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Lab Session 8: Selective and Differential Media‚ Unknown ID (Enzyme Based Tests) Oct. 25-31st (To be turned in PRIOR to start of recitation for lab 8) Name: _____ ________ Objective: Analyze microbes from last week. Understand the use of antibiotics on microorganisms. Gain more knowledge about selective media and differential media. Practice use of the catalase test‚ coagulase and the oxidase test. Observe microbial flora of the nose. Significance: Understand the use of Mannitol
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Survey of Nasal Carriage of Staphylococcus aureus in Microbiology 1 Students at RMIT Aim: To determine the carriage rate of Staphylococcus aureus in the nares of students taking second year microbiology courses at RMIT. Introduction: Carriage of S.aureus is important in hospital patients‚ preoperative patients‚ hospital staff‚ food handlers etc. because it carriage of S.aureus appears to play a key role in the epidemiology and pathogenesis of infection. S.aureus can cause localized and invasive
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