substance derived from algae and seaweed that has found considerable use in laboratories as a medium for bacterial growth. Seaweed is fairly common in oceans‚ with Gelidium amansii (a species of red algae heavily used for agar) usually found in East and Southeast Asian coasts. Coupled with differing nutrients‚ inhibitors and indicators‚ Agar plates can be utilised as both a selective and differentiating medium by allowing only bacteria with the required properties to flourish(2). By preparing a nutrient
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of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs. There are several sections to this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read in your lab manual about the following agar mediums: Blood Agar (pg 168)‚ EMB Agar (pg 170)‚ Mannitol Salt Agar (MSA)(pg 172) )‚ MacConkey Agar (pg 174)‚ and PEA Agar (pg 176) to answer the following:
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(1.0%‚ 1.5%‚ and 2.0%) of Hughes‚ et. al. (1958) Mineral Medium No. II. After ten (10) days of culture‚ the Trichome Length (TL)‚ Trichome Width (TW)‚ Sheath Width (SW) and Total Length of the Lyngbya filaments were measured from photomicrographs of the samples using Image Tool (Version 3.00) developed by the University of Texas Health Science in San Antonio (UTHSCSA). Lyngbya filaments in all cultures enriched with the Hughes medium obtained significantly higher TL and TW over those of the
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The identification of an unknown bacterial environmental isolate through a series of morphological‚ physiological and differential exercises. The purpose of the environmental isolate report is to learn what is necessary in order to take an unknown environmental isolate (EI) and identify it. This was achieved through a series of exercises that provided information on the morphological‚ physiological and biochemical traits of the EI which were then compiled and interpreted in order to make a presumptive
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lesions. Initially‚ infected tissue is water soaked but becomes necrotic (brown or black) in a few days. Often the lesions are surrounded by a halo of light green tissue. Spores appear as white velvety growth at the edge of the lesions‚ primarily at the underside of the leaf. It is this white growth that distinguishes Phytophthora leaf blight from several other foliar diseases. Often large amounts of sporangiospores are produced as 1-4 sporangiophores extend from the stomata at the underside of the
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tiny particles that appeared to be blood cells through the microscope. QUESTIONS: Compare your observations from the fresh yogurt slide and the prepared yogurt slide. Are there differences? Why or why not? The fresh yogurt appeared to have less growth than the prepared slide of yogurt. This is because the prepared yogurt was no longer gestating and showed an increase in bacterial numbers. Were you able to identify specific bacterial morphologies? Which
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Review for Microbiology Test #1 (Lesson 1-5) Lesson 1 What is the #1 killer of man worldwide? Heart Disease. What is the #3 killer in the US? Infectious Disease. What is the importance of MO in our world? List 6-8 reasons MO are important. Can’t live in Germ Free World‚ Keeps Economy running‚ Agriculture‚ Medication‚ Baking‚ Cosmetics‚ Paints‚ Fertilizers‚ Helps develop immune system‚ Decomposition of dead plants and animals to enrich the soil. What are the 4 groups of people most prone to
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414L Lab Report: SOC Medium I. Purpose: To make liquid and solid media for microbial growth II. Background: There are four different types of media used to grow microbes. They consist of Enriched media‚ Nutrient media‚ Selective media‚ and differential media. Enriched media is a growth media supplemented with complex biological molecules. This type of media is used for blood‚ coagulated blood‚ amino acids‚ etc. Nutrient media provides nutrients necessary for the growth of microorganisms. Selective
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inhibiting the growth of gram positive bacteria. The lack of growth from my bacteria indicates that my bacterium has negative result. Mannitol Salt Agar This test is used to observe the difference between gram positive staphylococci bacteria and to differentiate for mannitol fermentation. If a yellow media change is observed then the bacteria has the presence of acid by-products of mannitol fermentation. For my bacteria no change in the color of the media is observed but growth is. Therefore
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The antibacterial activity of the green synthesized silver nanoparticles from aqueous extract of Gum of fresh Coconut was tested by the disc diffusion method against an antibiotic‚ ampicillin(1µg/µl) as a standard drug. The antibiotic resistant bacteria viz.‚ Staphylococcus aureus‚ Bacillus subtilus‚ Escherichia coli and Klebsiella pneumonia were used for the measurement of antibacterial activity‚ and the plates were incubated at 370C overnight to know the zone of inhibition. 2.2. Cell Culture and
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