agar plates To distinguish the growth characteristics of microorganisms in various differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin sample. Gram iodine. Results: Starch agar: Special types of media: Type of medium Medium Appearance of medium and growth Selective and differential Eosin-methylene
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IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. It contains bile salts (to inhibit most Grampositive bacteria‚ except Enterococcus and some species of Staphylococcus)‚ crystal violet dye (which also inhibits certain Gram-positive bacteria)‚ neutral red dye (which stains microbes fermenting lactose)‚ lactose and peptone. QUALITY CONTROL Results after 24 hrs at 35º C Organisms ATCC Growth Colour Escherichia coli
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........................................................................................1 History of Microbiology and Culture Media ...................................................................................................3 Microorganism Growth Requirements .............................................................................................................4 Functional Types of Culture Media
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the mannitol salt were used to incubate a TSB broth to grow the gram-‐positive culture. The purity of this broth was tested using gram-‐staining technique. A circular colony from the TSA plate was used to incubate a TSB broth for gram-‐negative growth. Similarly‚ examining the morphology of the bacteria-‐using gram staining technique tested the purity of the both. After the isolation of gram-‐positive and gram-‐negative bacteria from unknown A‚ specific biochemical tests
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counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count‚ like pour plate method‚ spread plate method and most probable number method. The viable count is very specidic‚ as it represents the number of colony forming units (/g) or (/ml) of the sample
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polysaccharide agarose‚ and a heterogeneous mixture of smaller molecules called agaropectin. Throughout history into modern times‚ agar has been chiefly used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture medium for microbiological work. Agar (agar-agar) can be used as a laxative‚ an appetite suppressant‚ vegetarian gelatin substitute‚ a thickener for soups‚ in fruit preserves‚ ice cream‚ and other desserts‚ as a clarifying agent in brewing‚ and for sizing
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Samples from three different sites were taken from Mount Arabia in Georgia‚ and were analyzed in lab. The results from the data collected showed that greater microbial diversity was present in hairy cap moss than in lichen‚ with a greater percentage of growth for each colony. This could be the result of the mutual symbiotic relationship that bacteria share with hairy cap moss‚ a greater source of nutrients available for bacteria in hairy cap moss in soil than in lichen on bare rock‚ or due to the temperature
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antifungal activity of bacterial strain Paenibacillus elgii TS33 25. The nutrient agar medium was prepared‚ sterilized at 121ºC‚ poured into the sterilized peteriplates and allowed to solidify under aseptic conditions. After solidification bacterial strain Paenibacillus elgii TS33 was spot inoculated on nutrient agar medium and incubated at 37ºC for 48 hours. After incubation the molten potato dextrose agar medium containing the spores of test fungus‚ was spread on the same plate and reincubated at
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