Jennifer Hauss March 4‚ 2015 Bacterial Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three
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Optimal Growth Conditions and Generation Time for E. coli Introduction The goals of these exercises were to determine the optimal growing condition and the generation time of E. coli. Materials and Methods Please refer to the lab manual for a complete list of materials. To determine the optimal growth condition for E. coli‚ we had to test the environmental factors that could affect the growth. We tested the relative growth of E. coli in the presence and in the absence of oxygen. The results
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of Shiga toxin producing Escherichia coli O104: H4 outbreak‚ Germany 2011 The Escherichia coli O104: H4 outbreak of 2011 originated in northern Germany and lasted from May through to June of the same year. “The outbreak was characterized by several unusual features: a high incidence in adults (especially women)‚ a greatly increased incidence of the haemolytic–Uraemic syndrome (in approximately 25% of patients‚ as compared with 1 to 15% in previous outbreaks of Shiga-toxin–producing E.coli)‚ a predominance
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HAT 1 Task 3 A. Analyze the selected communicable disease outbreak by doing the following: Describe the communicable disease outbreak. Avian Influenza‚ also known as the Bird Flu‚ is an influenza A virus caused by avian‚ which means bird. These viruses occur naturally in some birds. Wild birds in all areas of the world get the viral infections in their intestines‚ but do not usually become ill from them. The avian influenza is quite contagious among birds and can even kill domesticated
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Catherine went to live with her aunt‚ Catherine Ray Greene. 19-year-old Catherine married Nathanael Greene on July 20‚ 1774‚ in Greenwich‚ Rhode Island after they had met at one of her aunt parties. Nathaniel was 14 years older than her at the time. Following their wedding‚ the couple moved to Nathanael Greene’s home in Coventry‚ Rhode Island‚ where they spent less than a year together before he was called into service. In the fall of 1785 the Greenes left their family and friends in Rhode Island and
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Graham Greene: A Catholic Novelist Henry Graham Greene (1904-1991) is famous around the world as a prolific writer. During his career‚ extending over sixty years‚ he wrote more than twenty novels‚ a collection of short stories‚ several plays‚ screenplays as well as books of travel writing‚ essays and film criticisms. It’s not an easy matter to deal with such a milestone in English literature that came through so many different periods and matured so much over the time[1]. One aspect of his personality
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Angelica Beatrice Y. Chong Prof. Mylene Andaya BS Biology Outbreak (1995) Outbreak is movie about a certain virus called Motaba that is highly contagious. Motaba is derived from the place where it originally came from‚ in Motaba River Valley‚ Zaire. From being a deadly virus that is not air-born it somewhat
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The effects of black tea on the growth of E. coli bacteria cultures Kierstin Barker‚ Melissa Bischak‚ Jackie Tyszkiewicz‚ Errin Enany September 25‚ 2012 Abstract: This study was carried out in order to investigate whether black tea has antimicrobial properties as stated in Steven Johnson’s The Ghost Map. If tea does have antimicrobial properties‚ then it could aid in warding off waterborne diseases. We believed that if black tea is steeped in boiled water‚ then the amount of bacteria exposed
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The Figure 4.20 shows the profile growth of Escherichia coli type K-12 which was cultured in 20 g/L of glycerol treated with supplements from a carbon source under anaerobic condition and the medium supplemented with 10 g/L of trypton. About 10% working volume of inoculums were added to the medium for the profile growth process. The effects on growth curves of strains of Escherichia coli were investigated by measuring the concentration of succinic acid produced and its optical density by using HPLC
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Growth Dynamics of E. coli in Varying Concentrations of Nutrient Broths‚ pH‚ and in the Presence of an Antibiotic Dvora Szego‚ Elysia Preston Darcy Kmiotek‚ Brian Libby Department of Biology Rensselaer Polytechnic Institute Troy‚ NY 12180 Abstract The purpose in this experiment of growth dynamics of E. coli in varying media was to determine which media produces the maximum number of cells per unit time. First a control was established for E. coli in a 1.0x nutrient broth. This was used to compare
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