Introduction The Gram stain is one of the most useful staining procedures in microbiology . It is one of three differential staining techniques‚ is used to identify divide bacteria into two groups: Gram-positive bacteria and gram-negative (Madigan‚ Martinko‚ Dunlap‚ Clark (2009). These staining reactions take advantage of the fact that cells or structures within cells display dissimilar staining reactions that can be distinguished by the use of different dyes (Brown 2009). A gram positive bacteria
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Staphylococcus aureus (S. aureus) is a gram positive bacterium that when looked at under a microscope it appears to be a cluster of what looks like purple circles. This shape is known as cocci. When grown on a TSA plate‚ Staphylococcus aureus appears to be yellow to opaque in color. S. aureus is known as one of the most resistant bacterium to multiple antibiotics and considered the most pathogenic. Everyone is susceptible to S. aureus with one way of transmission being from foods such as chicken
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Micro Biology Lab Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining. Gram Stains is used to identify and classify bacteria‚ it is a differential stain that allows you to classify bacteria as either gram-positive or gram-negative. The foloowing is a breif summerary of the gram staining procedure. Apply the primary stain Apply Mordant Apply decolorizing agent Apply the secondary stain Bacteria that
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Gram staining From Wikipedia‚ the free encyclopedia A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923‚ Gram-positive cocci‚ in purple) andEscherichia coli (Escherichia coli ATCC 11775‚ Gram-negative bacilli‚ in red)‚ the most common Gram stain reference bacteria Gram staining (or Gram’s method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). The name comes from its inventor‚ Hans Christian Gram. Gram staining
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
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The Staphylococcus aureus‚ a bacteria that is found in the nose‚ has been a leading cause of infection in hospitals around the world. This is an antibiotic-resistant‚ nosocomial pathogen‚ meaning that it’s acquired at hospitals. To test the idea that these infections have increased‚ eight hospitals participated in a study within the New York Area. The following are hospitals participated in this study: VA hospital‚ Kings County Hospital‚ Columbia University Medical Center‚ New York-Presbyterian Queens
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Foodborne Illness Short Answer Questions Staphylococcus • What is the infectious agent (pathogen) that causes this infectious disease? The pathogen that causes Staphylococcus is called Staphylococcus aureus. Staphylococcus aureus is also called Staph and is abbreviated to S. aureus or Staph aureus in medical literature. S. aureus is a bacterium that causes various infections. Staph is a commonly found on the skin and also in mucus membranes (mostly the nose and throat) of up to 25% of healthy
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Gram Staining Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells‚ which is the primary stain. Those that retain the color are grouped as Gram-positive‚ and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic‚ making this process useful for detecting infections
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The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
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