of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms: Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. Part 4: Direct Staining: Record your observations for each sample. Part 5: Indirect Staining: Examine the stained specimens and record your results. Questions: A. What are the advantages of using bleach as a disinfectant? The disadvantages? The advantages of using 70% alcohol? The disadvantages
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observations. It was hard to tell what I was looking at. There were a number of amoeba shaped cells of varying sizes. There were five darker areas circular in nature. There were also three very large‚ odd shaped areas with distinct edges. Part 4: Direct Staining: Record your observations for each sample. Slide One: There were two distinct clusters that were easily noted. All the cells were cocci. Some of the cells were huge while others were practically nonexistent. Slide Two: There were layers of cells
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contain cocci with direct staining. At direct staining plaque smear contain cocci and bacillus. Yeast with indirect staining contains cocci as well. On same picture bacteria are singled while on some pictures bacteria are in group and uneven clusters. Different type of stain plays role to differentiate the shape of bacteria. C: When we are talking about difference between direct and indirect stains‚ main importance is acidity of the dye. Dye that we are using in direct staining is basic. The colored
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Chapter 17 Louis Pasteur’s experiment illustrated that: microbes will not grow in a nutrient broth that has been sterilized unless air is allowed to enter the vessel through an opening. Living organisms can spontaneously generate from nonliving matter. True or False? False The scientists usually given credit for disproving the theory of spontaneous generation of bacteria are: Tyndall & Pasteur Miller and Urey’s experiments attempting to recreate the prebiotic environment produced a rich mixture
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Quantitative histological analysis of the laceration site by vimentin immunohistochemical staining 4 weeks after injury. Top panels (A)‚ vimentin immunohistochemical stain; lower panel (B)‚ area of scar tissue per field. Control (C)‚ muscles treated with IGF-1 (IG)‚ muscles treated with decorin (D)‚ and muscles treated with both agents (IGϩD); *P Ͻ 0.05. FIGURE 3. Quantitative analysis of scar tissue by trichrome staining. Scar tissue containing collagen (blue) was analyzed. Top panels‚ trichrome stain;
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Genetically Modified Organisms INTRODUCTION: The purpose of this lab was to identify if non-labeled food products are actually genetically modified foods. Before we could begin testing this theory we first had to gain an understanding about genetically modified organisms in general. This was rather easy because if you have been to any grocery store lately you have without a doubt seen products with labels saying "GMO-free" or even "contains only non-GMO ingredients." GMO actually stands for
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2.4 Mechanism Of Phosphate Solubilization: In contrary to Nitrogen soluble Phosphorous is not supplied to plants by atmosphere . Due to this reason the primary and secondary minerals and/or organic compounds are the source of P to great extent. In soil solution concentration of phosphorous is least as compare to other nutrients and ranges from0.001 to 1mg/ml Brady and Weil (2002 ).There are widely three categories of P compounds in soil: (i) compounds which are inorganic‚ (ii) organic compounds
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arising from the isthmus in front of the larynx • The gland is well vascularized‚ and the thyroid has one of the highest rates of blood flow per gram of tissue of any organ in the body. Anatomic considerations • The thyroid is made up of multiple acini (follicles). • Each spherical follicle is surrounded by a single layer of cells and filled with pink-staining proteinaceous material called colloid. Anatomic considerations • When the gland is inactive‚ the colloid is abundant‚ the follicles are large
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Tissues of the periodontium (Chapter 2) Periodontium * The tissues that surround‚ support‚ and attach to the teeth Components of the periodontium 1. Gingiva 2. Periodontal ligament 3. Cementum 4. Alveolar bone Function of the periodontium * To support the teeth and oral structures The gingiva * The visible component of the periodontium inside the mouth * Described as: pink‚ pink-red‚ blue‚ purple‚ or pigmented * It can appear much darker when melanin pigmentation
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stained if the dyer solution is used at the specific pH at which the reactive groups are fully ionized. To achieve full ionization of the reactive groups‚ some alcian blue methods begin with a rinsing of sections in the appropriate pH solution prior to staining with the dye solution. In general‚ strongly sulfated mucins react more consistently at low pH levels. Above a pH of 1.0 their reactions are variable. At a pH of 0.2 only strongly sulfated mucosubstances will be demonstrated‚ while weak sulfated mucins
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