different biochemical tests for the isolated organisim from the sputum sample‚ it was concluded that the unknown Gram positive organism was Streptococcus pyogenes. The sputum sample was first inoculated in the agar plates MAC‚ SBAP‚ CHOC and also a direct gram stain of the sample was performed. After a day of incubation‚ the MAC agar gave a negative result‚ therefore it is not a Gram-negative bacteria and non-lactose fermenting. The SBAP and CHOC showed growth of colonies. The CHOC agar had a mixed
Premium Bacteria Microbiology Staining
PART ONE: GENERAL BACTERIA IDENTIFICATION Review the dichotomous key in Figure A‚ the bacterial shapes in Figure B‚ and the Gram stain information below. You will use all three to determine to which major group unknown bacteria belong. Gram Stain Results: Purple = Gram positive Red = Gram negative Neither purple nor red = No cell wall (neither Gram positive nor Gram negative) Review Figure C on the following page. Use Figure C to identify the type of arrangement displayed by the unknown
Premium Bacteria Microbiology Gram staining
report discusses an experiment that was done to demonstrate the effects of lysozyme on populations of Gram positive and Gram negative bacteria. Bacteria have a cell wall composed of peptidoglycan that gives the wall its strength. Gram negative bacteria have and extra component of lipopolysaccharide (LPS)‚ that is stabilized with magnesium ions‚ to their cell wall that further protects them. When Gram positive bacteria are treated with lysozyme‚ the lysozyme breaks down the peptidoglycan‚ allowing the
Premium Bacteria Staining Microbiology
and was moved to the incubator for 48 hours at 35 degrees Celsius. The same procedure was used on all of the samples. Following the 48 hour incubation period‚ the Petri plates were observed for colony morphology‚ and the results were recorded. A Gram
Premium Gram staining Bacteria Microbiology
Since it was gram positive‚ I knew my organism had a thick peptidoglycan and no outer membrane. After finding out my organism B was gram positive‚ I did the catalase test on it. After adding the two drops of H202 and seeing bubbles‚ I ruled out Streptococcus lactis. My organism did have the catalase enzyme; meaning it was
Premium Bacteria Staining Gram staining
POLHN Microbiology Student Name: SOKOPETI FAKAOSIFOLAU. Assessment 6 – Gram negative bacilli 1. Which of the following is not true of Enterobacteriaceae? a. Gram negative bacilli b. Oxidase positive c. Reduce Nitrate to Nitrite d. Ferment glucose with acid production 2. Which of the following organisms does not belong to the Enterobacteriaceae group? a. Shigella flexneri b. Eschericia coli c. Proteus mirabilis d. Pseudomonas aeruginosa 3. Name the four species of Shigella
Premium Bacteria Microbiology Escherichia coli
is to distinguish and indentify an unknown bacterium. There are several tests that can help one eliminate and narrow down the options. The most useful test‚ and the very first one done‚ is a gram stain. This test will tell whether the bacterium is gram-positive or gram-negative. After the type of gram stain is identified‚ the tester has a wide array of differentiating tests at their disposal. Based on the results from these tests‚ and the numerous others that are available‚ one can accurately
Premium Bacteria Microbiology Meningitis
identification of a mixed culture of Gram-negative and Gram-positive bacteria 2. Author: Nick Fiore‚ University of Kansas‚ Biology 402‚ Fall 2014 3:00pm room 6040 3. Abstract: The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria. The Gram-positive bacteria were
Premium Bacteria Microbiology Gram staining
was in our number seven vial would become a battle we were willing to take on. As I began the test of deciding if our little bacteria friend was gram positive or negative‚ Jordan my science teammate‚ was putting together a smear plate. In as little as ten minutes we had discovered by the pink oval shapes we were observing‚ our microbe friend was a gram negative rod. We had narrowed our search down to five! We decided next we would do oxygen along with a motility test. Along with those‚ we did a fermentation
Premium Bacteria Microbiology Staining
Unable to read the Gram Stain at first it was later confirmed that Unknown #7 was a gram negative bacteria. When introduced to Starch Unknown #7 produced no zone of clearing showing that it did not contain the enzyme amylase. When introduced to the Lipid Unknown #7 did produce a zone of clearing indicating that it did contain the enzyme lipase. When introduced to Casein Unknown #7 produced no zone of clearing indicating that it did not contain the enzyme protease. When streaked onto the Gelatin‚
Premium Bacteria Gram staining Staining