Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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In March 10‚ the first procedure that was done was gram staining. The purpose of this procedure was to determine if the unknown bacterium is gram-positive or gram-negative‚ and also to determine the cell shape. Gram-positive cells will be purple while gram-negative cells will be pink. The methods for the Gram staining method first starts with a fixed smear of the bacterium‚ covered it with crystal violet for 30 seconds. Afterwards‚ it should be gently rinsed off with distilled water and be covered
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Review Sheet Exercise I: Survey of Higher Microorganisms: Protozoa‚ Fungi‚ and Helminths Protozoa (group of Kingdom Protista) 1. Amoeba a. nucleus- dark center of the cell b. food vacuole- They feed by taking nutrients into the cell by diffusion and packaging it into (clear circles spread throughout the cell) c. pseudopod- “false foot”; the motility results from the streaming of the protoplasm that forms the process 2. Entamoeba causes amoebiasis or amoebic dysentery‚
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shapes would distort. BM SC Negative Stain CULTURES: Bacillus subtilus Staphylococcus epidermis MATERIALS: Nigrosin‚ Clean slide‚ Distilled water‚ Sterile toothpicks FUNCTION: This technique is very useful in situations where other staining techniques do not show clear morphology or size. PROCEDURE: 1. Place a small drop of nigrosin near on end of the slide. Mix a loopful of broth culture in the drop. (When from a solid medium‚ add a loopful of water) 2. Draw a second slide
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Pets as carriers of soil inhabitant microbes into our homes GIZELL VASQUEZ Biology department‚ Pathogenic microbiology‚ Crosby Jones‚ Angelo State University‚ San Angelo‚ TX 76909 There are approximately 78.2 million owned dogs in the United States (1)‚ and according to the United States government the current population of the nation is 308.7 million. The portion of dogs to humans is 4:1. These statistics are of importance because these pets physiologically observed their environment by smell
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the plate‚ it could be seen that the unknown had growth and formation of colonies. The Gram stain was chosen next to determine if the unknown was gram-negative or gram-positive. After looking at the gram stain under the microscope with the oil immersion it could be determined that the unknown was gram-negative due to the fact that the bacteria was pink in color and rod shaped. After determining the unknown was gram-negative‚ other bacteria’s were able to be eliminated from the options. The Triple Sugar
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Trypticase Soy Agar plate to isolate individual colonies to be studied‚ tested and identified. After incubation of the individual TSA plates‚ the morphologies were viewed and noted and a Gram stain was completed on each individual bacterium‚ which will be referred to as Bacteria #1 and Bacteria #2. After the Gram reaction was determined on Bacteria #1 and Bacteria #2‚ different biochemical tests were done according to the dichotomous keys provided in the lab manual. All the tests were performed by
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* Length of L. lactis ranges from 0.5-1.5 micrometer (µm). * L. lactis does not produce spores (nonsporulating). * No capsule formation. * L. lactis grow in microaerophilic conditions * L. lactis are non motile. * L. lactis are Gram (+ive). * L. lactis are catalase (-ive). * L. lactis have low-GC content. * L. lactis are acid-tolerant * Optimum growth temperature is 30oC at pH 6.8. * L. lactis are not produce CO2 in MRS Broth. Colony Morphology: * No
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Introduction The Gram stain is one of the most useful staining procedures in microbiology . It is one of three differential staining techniques‚ is used to identify divide bacteria into two groups: Gram-positive bacteria and gram-negative (Madigan‚ Martinko‚ Dunlap‚ Clark (2009). These staining reactions take advantage of the fact that cells or structures within cells display dissimilar staining reactions that can be distinguished by the use of different dyes (Brown 2009). A gram positive bacteria
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Review for Microbiology Test #1 (Lesson 1-5) Lesson 1 What is the #1 killer of man worldwide? Heart Disease. What is the #3 killer in the US? Infectious Disease. What is the importance of MO in our world? List 6-8 reasons MO are important. Can’t live in Germ Free World‚ Keeps Economy running‚ Agriculture‚ Medication‚ Baking‚ Cosmetics‚ Paints‚ Fertilizers‚ Helps develop immune system‚ Decomposition of dead plants and animals to enrich the soil. What are the 4 groups of people most prone to
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