slide before staining? Differentiate between a differential stain and a special stain (how are they different‚ don’t simply identify the types of differential stains and special stains). What is the role of the Gram’s iodine in a Gram stain? What color would all bacteria become at the end of the staining procedure if the iodine in the gram step was omitted? What is the role of the alcohol wash in the Gram Stain? What color would all bacteria become at the end of the staining procedure
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate the bacteria. The different staining reagents are: crystal violet‚ grams iodine‚ acetone-alcohol‚ and grams safranin. Under the microscope it was a pink color‚ which means Gram Negative. Also‚ the shape was a rod. 2. KLIGER’S IRON
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inoculating loop or needle‚ Bunsen burner‚ glass slides‚ lens paper‚ bibulous paper‚ regular tap water‚ along with reagents Crystal violet‚ Gram’s Iodine‚ 95% ethyl alcohol‚ and safranin. The first test I conducted was the gram stain. In preparation for the actual gram staining process I had to make a smear. To do this‚ I acquired a clean glass slide. I then placed one drop of water onto the center of the slide. Using the flame from my Bunsen burner I sterilized my loop by passing the malleable
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bacteria and applying it to the results. This helps to slowly eliminate any bacteria that do not correspond with the results. I obtained tube number twelve to run various tests on. There are only two microorganisms‚ one is Gram positive and one is Gram negative‚ inside the test tube. The Gram positive possibilities are to be narrowed down from Bacillus cereus‚ Clostridium perfringens‚ Corynebacterium xerosis‚ Enterococcus faecalis‚ Streptococcus agalactaie‚ Staphylococcus aureus‚ and
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cross walls). Some have slow gliding locomotion. (Rainis 43) When looking at a bacterial sample under a microscope‚ the experimenter has to stain the bacteria first. The stain serves to define the bacterial cells. All bacteria are either gram negative or gram positive. To stain a sample first the scientist has to “set” the sample. Setting the sample is the basis for all stains. To “set” or simple-stain a sample of
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Activities/Tasks/Responsibilities | Problem(s) Encountered | Action(s) Taken | Personal Reflection | BacteriologyJuly 4- July 31 | Receiving of specimen‚ Logging‚ Processing of specimen‚ Inoculation into media‚ biochemical testing‚ identification of organism‚ gram staining‚ AFB staining‚ releasing of results‚ culture sensitivity testing. | No major problems were encountered during my rotation for Bacteriology. Minor problems like unfamiliarization of SOP’s and other procedures are encountered. | Asking proper procedures
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particular order. Name this Procedure Identify the two types of bacteria present by shape and gram stain. In a gram stain what is the primary stain? The mordant? The counterstain? How does this differ from a simple stain? Identify the shape and gram stain of the bacteria present in this gram stain. Identify the shape and gram stain of the bacteria pictured. Think on this one! Name the gram stain and shape of the bacteria pictured. Name the genus of the organism pictured. Note the size
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tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I had to run three experiments in all before I
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Abstract: It is important to be able to identify pathogenic bacteria that may be causing harm. Tomato crops can be affected by several different pathogenic bacteria. By using Koch’s postulates‚ it was determined that Pseudomonas syringae was the bacteria causing rot. There are four criteria that must be met when using Koch’s postulates. They are that the organism must be fund in all infected‚ the organism must be isolated in pure culture then once reinnoculated in a healthy host‚ must cause the
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