MICROBIOLOGY SECTION The microbiology section aims at studying yu ailments and diseases from the isolation of suspected causative organisms. The processes involved in the isolation of these organisms include; culturing‚ staining‚ microscopy and sensitivity tests. Samples collected for examination include; stool‚ blood‚ sputum‚ urine‚ vaginal swab‚ wound swab and wound biopsy. Bacterial Culturing and sensitivity Cultures are carried out to isolate suspected organisms from a sample. There are different
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Microbiology report Gram stain Objectives: The aim of this experiment was to be able to stain bacteria correctly and be able to see it under the light microscope. In 1883 Hans Christian Gram discovered an important staining method that is used extensively today. The stain is called the Gram Stain. This experiment was done in order to differentiate microbes into two basic groups: Gram positive microbes and Gram negative microbes. The purpose of this experiment was to learn the gram staining method and to
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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Robert Suran May 1 2013 Microbiology Abstract Over a three week time span I was able to successfully identify an unknown microorganism by combining the results of several tests. The first test was the Gram stain test which showed the microorganism was Gram positive and rod shaped. Using an Unknown Identification Flowchart‚ I proceeded to inoculate a Starch plate. In week 2‚ I analyzed the Starch plate and was able to determine that the results for Starch Hydrolysis were negative
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Microscopic observations were performed on four different microscope slides. Three of these observations were gram stains and the last observation was of an endospore staining. The unknown bacteria samples for the endospore stain were taken off of a nutrient agar plate and stained using the Bartholomew and Mittwer’s method. Gram stain samples were taken off of tryptic soy agar and stained using the gram stain procedure that is listed in the Willgoh and Bleakly manual (2). Physiological Tests All biochemical
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Question 1 of 12 2.0 Points All of the following are considered microbes except A.viruses B.bacteria C.protozoa Correct D.worms Answer Key: D Question 2 of 12 2.0 Points Microbiologists investigate problems by designing and carrying out experiments. What is true about the scientific method? A.a hypothesis is the definitive explanation to account for the observation and therefore does not need to be tested B.a prediction is the factor that can change
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1995 Del Mundo‚ Layah Basella rubra as a biological stain Experimental The alugbati extract turned out to be favorable stain for plant nuclei and organelles especially for Hydrilla verticillata and as substitute for crystal violet or safranin in Gram staining. The extract stained the bacteria red-orange and the plant nuclei‚ and the cytoplasm red and light pink‚ respectively. The study is limited to the ability of Basella rubra (alugbati) to be used as a biological stain 2011 Gel-O-Fury- Saavedra‚
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and one result was inconclusive. The focus of this paper will be on the description of the just one of the three bacteria and some of its effects on humans. The first test done was a differential stain (gram stain) to determine the unknown microorganism cell shape and arrangement. The gram staining is done by first putting a sample of the unknown bacteria on a slide and it was heat fixed taking care not to over or under fixed the slide. The slide was flooded with crystal violet and let stand for 30
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Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial colony was isolated form Mycorrhizal spores‚ and further tests done on that colony. Sub culturing was done after each week to ensure
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testing in order to determine the identity of the unknown organism. The tests performed to identify the unknown organism included Gram Staining‚ Fluid thioglycollate/aero-tolerance test‚ Methyl Red-Vogues Proskauer‚ SIM test‚ Lysine Decarboxylase test‚ Lactose Fermentation test‚ Simmons Citrate test‚ and TSI test. The Gram staining technique showed that the organism was Gram negative and bacilli shaped. The fluid thioglycollate test showed that the organism was a facultative anaerobe‚ meaning ATP can
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