Gram Staining Introduction Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria
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Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on the
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Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. It is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls. Gram positive bacteria (which show up purple after the gram staining process) have a very thick layer of peptidoglycan where gram negative bacteria (which show up pink after the gram staining process) have a much thinner layer of peptidoglycan. One thing
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Bacteria Classification By Gram Staining THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Objective: To test a sample of laboratory prepared bacteria and categorise it according to Christian’s gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics. Introduction: Bacteria
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Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased‚ not the writing. The purpose of scientific writing is not to entertain‚ but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports. Scientific writing is typically written in the passive voice. The
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Stainin g What is Cellular Staining? Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that cells
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Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus‚ Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded‚ clusters‚ singly
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Introduction The Gram stain is one of the most useful staining procedures in microbiology . It is one of three differential staining techniques‚ is used to identify divide bacteria into two groups: Gram-positive bacteria and gram-negative (Madigan‚ Martinko‚ Dunlap‚ Clark (2009). These staining reactions take advantage of the fact that cells or structures within cells display dissimilar staining reactions that can be distinguished by the use of different dyes (Brown 2009). A gram positive bacteria
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MBK – Lab Report Differential Staining Questions: A. What is a differential stain? How is it different from a simple stain? 1. A differential stain is a stain that differentiates between gram-positive and gram-negative bacteria. Bacteria that stain purple are considered gram-positive and those that stain pink are considered gram-negative. 2. A simple stain is a stain that reacts with all microbes in and identical fashion. They are used to increase contrast. 3. They
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1 Aim: To successfully identify and differentiate Neisseria Species and Moraxella catarrhalis; i. detail the microscopic and cultural characteristics of the Neisseria spp. and Moraxella catarrhalis‚ ii. differentiate the Neisseria spp. and Moraxella catarrhalis associated with humans‚ iii. describe the isolation media used for Neisseria gonorrhoeae and give reasons why they are used‚ iv. recognize gram-negative diplococcic in urethral and cervical smears‚ and‚ v. identify:
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