hyphal cells. d. True hyphae- Fundamental microscopic units of fungus‚ tube-like projections with no constictions at the cell wall. The cell walls remain parallel with no indentation. 3. Describe the appropriate specimen collection procedures‚ staining methods‚ and culture techniques used for isolation of yeast. Collection procedures Specimen of choice include respiratory secretions‚ hair‚ skin‚ nails‚ tissue blood or bone marrow and CSF. Swabs are inadequate. CSF: Concentrate by centrifugation
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INTRODUCTION Bacillus anthracis has recently become popular as the causative agent of anthrax infections. Belonging to the bacillus species‚ the bacterium is considered‚ along with Bacillus cereus‚ to be of extreme medical importance. Until recently‚ the condition caused by the bacterium was rarely heard of. Although the bacterium is naturally found in the soil‚ its rare occurrences in humans have only been observed in those working with livestock or imported livestock products‚ therefore the
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make easier manipulation on the bacteria for various purposes such as for medical‚ research‚ developmental‚ and biotechnicalfieldsThe cell wall is the basis for classification of bacteria according to the Gram stain. Accordingto the chemical make up of bacterial cell wall‚ a staining procedure‚ Gram stain‚ helps usclassify bacteria into two subgroups‚ The cell wall can stain either positive or negative‚depending on its chemistry. If the bacteria stains positive it will retain a purple/blue color. If
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Identifying the Unknown Bacteria: Bacillus cereus Brittany Moy Coastline Community College Abstract Bacillus cereus is a Gram-positive‚ facultative aerobic‚ spore-forming‚ rod-shaped bacterium that is widely distributed among the environment. Introduction The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria
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Differential Staining Purpose: The purpose of this experiment was to become familiar with subtypes of culture media and the uses for each‚ learn and employ the streak and pour dish techniques‚ and generate a pure culture of a specific organism. Set Up: For this experiment I needed: 1 Distilled water‚ 1 Paper towels‚ 1 10%-bleach or 70% alcohol solution‚ 1 Zip bag‚ 1 Pan to heat agar‚ 1 Isopropyl alcohol (rubbing alcohol)‚ 1 Cultures: S. epidermidis and L. acidophilus‚ 1 Gloves‚ Disposable
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is identified as Bacillus cereus. Bacillus cereus is a bacterium that grows best around 30-37°C. B. cereus cannot grow in high acidic‚ below pH of 4.5‚ or high alkaline environments of a pH above 9.3. You are likely to find this organism in the soil. This bacterium is able to form spores and therefore can withstand very harsh environments. This bacterium is commonly associated with food borne illnesses. To cause illness a large number (approximately 1 million bacteria per gram of food) of bacteria
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Lab Practical One Report Unknown Bacteria Staining Gram Acid Fast Spore The first practical for microbiology was staining of an unknown bacteria. Professor Goode provided an unlabeled bacteria in which each individual were to conduct gram‚ acid fast‚ and spore staining tests from memory. When the gram staining test was conducted a positive results was obtained. When views on oil bacteria appeared purple and rod-like. I noted this result and looked at the lab key to see what the results narrowed
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Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
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Microbiology Coursework: Bacillus cereus After investigation following on outbreak of food poisoning at a pizza restaurant‚ it was found that all suffers had consumed a portion of side salad from the self-service salad bar alongside their main dish. Subsequently‚ this was further traced to a rice salad. Environmental Health Officers investigating this outbreak suspected it may have been caused by Bacillus cereus (B. cereus). The presence of large numbers of B. cereus in a food is indicative
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Title: preparation of films for staining Objective: to produce a thin smear of bacteria adhering to a clean microscope slide as preparatory to staining. Procedure: A. From broth cultures 1. Inoculating loop was sterilized using Bunsen burner and let to be cool before use it to obtain bacterial suspension from the tube. 2. The bacterial then placed in the center of the clean microscope slide. It also speared to produce thin films. 3. The films are set to air dry before wafting the slide gently
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