Gram Staining Introduction Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria
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Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on the
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Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. It is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls. Gram positive bacteria (which show up purple after the gram staining process) have a very thick layer of peptidoglycan where gram negative bacteria (which show up pink after the gram staining process) have a much thinner layer of peptidoglycan. One thing
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Bacteria Classification By Gram Staining THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Objective: To test a sample of laboratory prepared bacteria and categorise it according to Christian’s gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics. Introduction: Bacteria
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medicine but there is still yet to be a perfect ideal antibiotic. In this experiment six antibiotics will be tested to determine which is the most effective on gram-negative bacterium Escherichia Coli and gram-positive bacterium Bacillus Cereus. In previous class experiments‚ gram-staining was conducted to determine the qualities of gram-positive
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Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased‚ not the writing. The purpose of scientific writing is not to entertain‚ but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports. Scientific writing is typically written in the passive voice. The
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Stainin g What is Cellular Staining? Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that cells
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Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus‚ Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded‚ clusters‚ singly
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The Identification of Bacillus Badius [pic] Hannah Melanchuk Dr. Robert Suran May 1 2013 Microbiology Abstract Over a three week time span I was able to successfully identify an unknown microorganism by combining the results of several tests. The first test was the Gram stain test which showed the microorganism was Gram positive and rod shaped. Using an Unknown Identification Flowchart‚ I proceeded to inoculate a Starch plate. In week 2‚ I
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STAINING FOR BACTERIAL ISOLATION Submitted by: Cherry Luz L. Rezaga Submitted to: Dr. Tomas J. Fernandez Jr. CRITERIA OF REPORTING 1. Thorough research (20%) 1.1. Number of literature cited more than five (5) 1.2. Completeness of the topic (5) 1.3. Principle discussed (5) 1.4. Literature should be cited in the text (5) 2. Confidence (20%) 2.1. Eye to eye contact (5) 2.2. No reading (5) 2.3. Proper handling of visual aids (5) 2.4. Magnified voice (5) 3. Appropriateness of visual
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