Part B: Gram Stain Preparation: Watch the following video: The Gram Stain (4:15 minutes) Procedure: Include the answers to the questions below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram positive
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Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
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culture‚ I immediately made a streak plate and started the Gram Stain protocol1. In the first two slides‚ I made and stained‚ my results were ambiguous. Without putting the slide into the microscope‚ I noticed one was pink and the other purple. To resolve it‚ I discarded my slides and started again and was extremely careful in proceeding the Gram Stain. I concluded that my unknown A was gram-negative bacilli. Using the dichotomous key for gram-negative bacilli I started the protocol for the lactose
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Report Experiment Tittle Gram stain Experiment Date 24October2014 Submission Date 31October2014 Students Name Tittle Gram stain. Objectives Aim of this experiment is to differentiate between the two major categories of bacteria ‚ gram positive and gram negative. Through this experiment‚ gram staining skills develop. More understanding the types and morphology of bacteria. Expected experimental result‚ Escherichia coli (E.coli ) is a negative gram bacteria which stain pink colour ‚ while Staphylococcus
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Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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Gram Stain: The important part of this experiment is being able to determine a bacterium based on its cell wall structure. It also helps indentify if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin
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Lab 4: Cell Structures and the Gram Stain Purpose: The purpose of this lab is to familiarize the student with the use of a compound microscope and the Gram Stain test. Students will review a brief video and slides to introduce the compound microscope. Students will also view a video about the Gram Stain procedure and then answer questions related to it. Hypothesis If I look at the slides I will see where the cell wall is and what lies inside it and in the Gram Stain if I watch the video I will
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Amanda Lab Report 1 Microbiology Laboratory Title – Differentiating Organisms using the Gram Stain Introduction The experiment conducted was based upon the known attributes of two different groups of bacteria‚ those that are gram positive‚ and those that are gram negative. Using a specific staining procedure‚ it is possible to differentiate the two types under a microscope The gram stain method of differentiation is possible because of differences in the cell membrane between the two categories
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Gram Staining Introduction Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria
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Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on the
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