Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
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virus too small to be seen without a microscope (smaller than 0.5mm) Misconceptions of Microorganisms * All microorganisms are pathogenic (disease producing) FALSE * The majority of bacteria are harmless and beneficial TRUE Aspects of microbiology * Basic biological science * About living cell and how they work * About microbial diversity and their evolution * Applied biological science * What microorganisms do; their role in the nature? * Causative agents
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Part B: Gram Stain Preparation: Watch the following video: The Gram Stain (4:15 minutes) Procedure: Include the answers to the questions below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram positive
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Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli Proponents: John Rovic P. Ngo Lester T. Almarez Trisha Mae N. Reyes August 2013 In Partial Fullfillment of the Requirements in Research II-B Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli A Science Research Project Presented to The Faculty of Science and Technology Mr.June Hayden Sinson Research Adviser
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Introduction:The objective throughout the gram staining experiment was to classify bacteria through their chemical nature as well as the physical structure of their cell walls. Through this experiment one would be able to observe either a thick or thin layer of peptidoglycan by the appearance of the bacteria staining either gram-positive blue-purple or gram-negative pink. Question: Is there a subsequent effect of the bacteria when decolorizing with alcohol? Hypothesis: If there is either a removal
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What Is Microbiology? Microbiology is the study (logy) of very small (micro) living (bio) things. Microbiology is the study of microorganisms. These "bugs" include: bacteria (that’s the Latin plural for bacterium); viruses (that’s the non-Latin plural for virus - virii sounds weird‚ so I don’t say it); and‚ fungi (that’s the Latin plural for fungus - which by now you have guessed‚ or already knew‚ and may not be all that interested to know‚ anyway). Microbiology is actually made up of several
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the product can be stained with the gram staining. Gram staining is the method of differentiating bacteria into 2 large groups of Gram-positive bacteria and Gram-negative bacteria based on their cell wall. Most of the bacteria or yeast in the starter cultures used are Gram-positive. Gram staining requires 4 types of solutions used in the procedure: crystal violet‚ mordant‚ alcohol and safranin. The Gram-positive microbes will have blue stain while Gram-negative stains pink after the procedure. Methylene
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Unknown Report Emily Juhl Section – 3 April 15‚ 2013 Unknown #8 Family: Enterobacteriaceae Genus: Serratia Species: Marcescens Abstract Microorganisms are constantly finding ways to resists to antibiotics. For this reason‚ it is important to test and observe unknown organisms in the lab to continually improve the health and well being of society. The objective of this report was to first isolate a single colony of an unknown culture on a LB agar plate. From
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Theodor Wilhelm Engelmann was a German microbiologist whose 1882 experiment measured the effects of different colors of light on photosynthetic activity and showed that the conversion of light energy to chemical energy took place in the chloroplast. In 1881‚ he observed the movement of bacteria towards the chloroplasts in a strand of “Spirogyra” algae. Engelmann hypothesized that the bacteria were moving in response to oxygen generated by the photo synthetically active chloroplasts in the algae.
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The Effectiveness of Antimicrobial Agents Bacteria: Clearasil and Proactive Veronica Hillgren 11/16/12 FRI AM Question/ Hypothesis The purpose of my experiment is to find and compare the efficiency of Proactive™ and Clearasil™ acne products on certain bacteria. For myself‚ Clearasil™ works better than Proactive™ does when exposed to my pores‚ which are infested with bacteria. However‚ Proactive™ is widely known and has a large amount
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