Microbiology 20 Laboratory Final Exam Guidelines The laboratory exam will be given on Tuesday December 4th (sections 0375 and 3252) or Thursday December 6th (section 3253) and consist of an open book portion given in CMS 105 followed by a practical portion (closed book) to be given in the lab (CMS 002). You will not need a Scantron. For the open book test‚ be sure to bring any resources you may need including your lab manual‚ lab notebook‚ safety guidelines and handouts. For the practical portion
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Terms to Know Decomposer - Breakdown of dead matter and wastes into simple compounds Prokaryote – No nucleus - microscopic‚ unicellular organisms‚ lack nuclei and membrane-bound organelles Pathogen - Microorganisms that do harm Eukaryote - unicellular (microscopic) and multicellular‚ nucleus and membrane-bound organelles alcohol - archaeobacteria - Prokaryotic single-celled organisms of primitive origin that have unusual anatomy‚ physiology‚ and genetics‚ and live in harsh habitats;
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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1. Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure? No‚
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Gram Staining Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells‚ which is the primary stain. Those that retain the color are grouped as Gram-positive‚ and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic‚ making this process useful for detecting infections
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MICROBIOLOGY What is the purpose of general growth media‚ selective media‚ and differential media? Give examples of each. General Purpose Media General Purpose media is designed to grow most organisms and do not contain growth inhibitors. Standard Methods Agar and Blood Agar Bases are examples of general purpose media. Differential Media Differential media contain a component that allow an observable change when a specific chemical reaction takes place. Simmons Citrate Agar is an example
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1 Student: ___________________________________________________________________________ 1. Extant microorganisms are organisms from the fossil record that are no longer present on Earth today. True False 2. All cellular organisms can be placed into one of three __________‚ which include the Bacteria‚ Archaea‚ and the Eukarya. ________________________________________ 3. Archaea are cellular organisms that have unique cell membrane __________. ________________________________________
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Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
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Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
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References: Alonzo‚ C. 2008. A Laboratory Manual of Small-Scale Experiments for Independent Study of Microbiology. Englewood‚ CO‚ US: Hands-On Labs‚ Inc. OSMOSIS
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