Arielle Fisher GFP Lab November 16‚ 2011 Figure 1 A. B. C. D. Figure 1. Confocal images at 400x magnification of HeLa cells to locate GFP activity. HeLa cells were (A) tagged with Green Fluorescent Protein (GFP) (B) labeled with GFP and 14.13 µl Dexamethasone (C) tagged with GFP fused to the glucocorticoid binding
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amount of protein‚ and percent yield and an increasing trend for specific activity and fold purification. These trends come about naturally when performing multiple purification steps. To determine the success of each purification step‚ the more important factors to look at are the total activity units‚ total protein amount‚ and percent yield. Looking at our purification table (table 7) our purifications were not very successful. While our total protein followed the normal decreasing trend‚ as
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The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE Abstract The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine
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DNA extraction and purification is an essential tool in understanding the biological basis and significance of a eukaryotic cell. Its role is pivotal to numerous scientific applications ranging from basic science research to applied research. Hence‚ DNA is found in all living organisms. Fragaria x ananassa‚ also known as a strawberry has been widely used as one of the many biological models in studying DNA structures due to its accessibility. Strawberries are octoploid which contain large genomes
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mummion assignment Task 1 – purification Aim We were given a sample of mummion in the form of dried mud from a lake; the aim of the practical was to purify the sample and separate it from the insoluble impurities into the form of mummion salt. Context A long time ago mummion was used to preserve the bodies of ‘mummies’ in ancient Egypt‚ mummion along with other naturally occurring preservatives was used to treat the organs before the bodies were wrapped. This purification practical happens very often
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LAB REPORT FOR EXPERIMENT #2: PURIFICATION OF ACETANILIDE BY RECRYSTALLIZATION Your name TA’s name Your Partner’s name Lab Section OBSERVATIONS: A. SELECTING A RECRYSTALLIZATION SOLVENT | |Solubility Test (cold) |Solubility Test (hot) | |Water |insoluble |soluble | |pet ether |insoluble
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Colegio de San Juan de Letran College of Liberal Arts and Sciences Experiment #3 SEPARATION AND PURIFICATION OF ORGANIC COMPOUNDS: CRYSTALLIZATION Gomez‚ Paola Anne M. DOP: 2 July 2013 Student no. : 4120403 DOS: 9 July 2013 Group: Carcinogenic Remarks: _____________________________________________________________________ Engr. C. D. Sanchez Instructor THEORETICAL DISCUSSION Crystallization is a technique which chemists use to purify solid compounds. It is
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Government-Furnished Property GFP What is Government-Furnished Property and Why do I care about it? Government-Furnished Property and You ▪ What is GFP? ▪ When do we provide GFP? ▪ Who is responsible for the GFP? ▪ The Government retains title to the GFP until it is properly disposed of as authorized by law and regulation. Government-Furnished Property Defined ▪ GFP is property in the possession of‚ or directly acquired by‚ the Government and subsequently furnished to the contractor for performance
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The Purity and Purification of Solids Recrystallization ABSTRACT In this experiment‚ impure adipic acid is purified though recrystallization. The impure adipic acid is dissolved in a solvent. After cooling it is ran through a vacuum filter to separate any moisture. The dry crystals are then weight for percent yield and the melting point range is taken to test the purity. There were two different trials ran in this experiment. The difference between the two was the addition of charcoal in
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