well their ability to be modified to express proteins of interest. A pGLO plasmid contains genes for the green florescent protein (GFP) as well as the gene for ampicillin resistance known as beta-lactamase. It also contains a gene regulation system (operon) that has the ability to control expression of the GFP gene in transformed cells known as araC. The source of GFP is naturally founds within a
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Experiment #4A- A Separation and purification Scheme Amena Alkeswani‚ PHCC Dhanlal Murally‚ PHCC September 27‚ 2012 Abstract: There are millions of different organic compounds. Most of them are found in mixtures and in order to achieve a pure form they need to be separated‚ isolated‚ and purified. However‚ there are endless numbers of possible mixtures‚ which make it impossible to have a pre-designed procedure for every mixture. So chemists often have to make their own procedures. The purpose
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African Diaspora and the World After reading the short story “Diaspora” by Joanne Hyppolite‚ I got a vivid understanding of how diaspora can have an effect on one’s identity. Over the past month we have studied many theories and concepts concerning race‚ gender‚ and politics. There are three theories that capture the essence of Joanne Hyppolite’s worldview as a Haitian growing up in America: intersectionality‚ identity‚ and diaspora. Individuals oftentimes experience the theory of Intersectionality
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Introduction: The method for purifying solids is done by observing the melting point. This is important because if there is an impure substance the melting point would be depression and the melting range would be broader. By finding the melting point of a known substance one can then observe an unknown substance’s melting point to see if the points are the same and try to figure out the unknown. Experimental Section: The first known substance we used was Biphenyl. 68 degrees Celsius is the
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Expression and Purification of Human Platelet-Derived Growth Factor Beta (PDGF-B) Rachel C. Hermina-Stewart* and Elsie I. Parés-Matos† *Industrial Biotechnology Program‚ †Department of Chemistry University of Puerto Rico-Mayagüez Campus rachel.hermina@upr.edu December 14‚ 2009 ABSTRACT. Platelet-Derived Growth Factor (PDGF) is one of many proteins that regulate cellular growth and division. The PDGF family consists mainly of five different isoforms called as PDGF-A‚ PDGF-B‚ PDGF-C and PDGF-D. Three
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will broaden range &may increase melting point Lab Questions 1. Define the following A. Melting point B. Intermolecular forces C. Hydrogen bonding 2. Arrange the following IMF according to there strength Dipole-Dipole interaction forces‚ Hydrogen bonding‚ Van der Waals forces 3. How does the atmospheric pressure affect the melting point? 4. What is the purpose of determining the melting point in this lab? 5. Why is this method not used for finding the melting
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introduction of new genetic information which is merged into the organism’s genome. Bacterial transformation is a type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab‚ the engineered pGLO plasmid is integrated into E. Coli bacteria‚ and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia coli with plasmids‚ 1983). To see the reaction of this plasmid on the
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Back Laboratory Program SAFETY IN THE CHEMISTRY LABORATORY 786 Pre-Lab Extraction and Filtration 790 1-1 Mixture Separation 792 1-2 Water Purification 794 3-1 Conservation of Mass 798 4-1 Flame Tests 801 Pre-Lab Gravimetric Analysis 828 13-1 Separation of Pen Inks by Paper Chromatography 830 13-2 Colorimetry and Molarity 834 14-1 Testing Water 838 804 7-1 Separation of Salts by Fractional Crystallization 806 7-2 Naming Ionic
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1. Why are spindle fibers important for mitosis? (5 points) Spindle fibers work as a path for chromosomes to travel(Campbell‚ 2009). 2. State the four bases that make up DNA. (4 points) a. (A)-Adenine b. (G)-Guanine c. (T)-Thymine d. (C)-Cytosine (Campbell‚ 2009). 3. What are the two base pairs? (2 points) a. AG b. CT 4. Answer the following questions: a. Define the term crossing over. (3 points) The phase in meiosis where chromosomes exchange segments of genetic materials (Campbell‚ 2009). b. Explain
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