Introduction: Bacteria are microscopic‚ single-celled organisms. Their genetic information is encoded in one large chromosome. It can also be found in plasmids which are small circular pieces of DNA that contain important genetic information for the growth of bacteria. In nature‚ this information is often a gene that encodes a protein that will make the bacteria resistant to an antibiotic. The reason for this protein being made within the bacteria is because of how bacteria usually grow in the same
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Specific Heat: The specific heat is the amount of heat per unit mass required to raise the temperature by one degree Celsius. The relationship between heat and temperature change is usually expressed in the form shown below where c is the specific heat. The relationship does not apply if a phase change is encountered‚ because the heat added or removed during a phase change does not change the temperature. Q=mcTDelta Abstract: In this experiment we are trying to determine the
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Transport of vesicles facilitated by kinesin and the effects of mutations in nerve transmission in Caenorhabditis elegans Introduction A nerve cell is made up of three main structures: the soma‚ the axon and the synapse. When a nerve cell receives a signal‚ a protein called kinesin travels anterograde along the axon and guides the synaptic vesicles until it reaches the synapse. This triggers the release of the neurotransmitters stored within the vesicles. Once released‚ the neurotransmitters then
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Society of Plant Biologists Dual Role for Tomato Heat Shock Protein 21: Protecting Photosystem II from Oxidative Stress and Promoting Color Changes during Fruit Maturation Inbal Neta-Sharir‚a Tal Isaacson‚b Susan Lurie‚c and David Weissa‚1 a Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture‚ Faculty of Agricultural‚ Food‚ and Environmental Quality Sciences‚ Hebrew University of Jerusalem‚ Rehovot 76100‚ Israel b Department of Genetics‚ Hebrew University of Jerusalem‚ Jerusalem 91904
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Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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main purpose of the experiment is to investigate heat transfer processes using the tubular heat exchanger which is a specific type of heat exchangers. Moreover‚ the primary goals are to compare the two types of flow cases‚ co-current flow and countercurrent flow as well as taking the analyses of their temperature changes through the graphical curves and observing the heat transmission via the operating apparatus. The working principles of the tubular heat exchanger is analyzed and the fluid temperate
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Module : Heat Transfer – Free Convection and Radiation Laboratory Date : 22nd March 2012 CONTENTS INTRODUCTION 3 AIMS & OBJECTIVES 3 Objectives 3 To investigate Free Convection and Radiation 3 Theory 3 EXPERIMENT 3 Apparatus Used 3 Procedure 4 RESULTS‚ CALCULATIONS‚ OBSERVATIONS & CONCLUSIONS 5 Observations During Tests 5 Table 1 5 Table 2 5 Calculations 6 Calculating Power (Watts) 6 Calculating Heat Transfer Emissivity (Ɛ) 6 Emisssivity of a black body
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Lab Report (Scientific Paper) 2: Bacterial Transformation;DNA Extraction Part I & II:Total Genomic Extraction & Plasmid Extraction;Electrophoresis By:Chris Foster Abstract: We conducted three experiments that included a Bacterial Transformation‚ a two process DNA extraction‚ and a final procedure using gel electrophoresis. The Bacterial Transformation lab was performed to prepare the plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities
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Calorimetry and Specific Heat Tessa Williams Chemistry 111 11/13/13 Abstract: In this experiment‚ the specific heat and the density of an unknown metal was determined in order to identify the unknown metal. The average specific heat of the unknown metal was 0.197˚C and was determined using a calorimeter. The density of the unknown metal was 6.57 g/mL and was determined using a cylinder and displacement. Using the specific heat value of the unknown metal and its density‚ it
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Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can
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