(mcΔT)Substance = - [(mcΔT)Water + (CΔT)Calorimeter] Materials: Coffee-cup calorimeter Water Safety Goggles Thermometer Lab Apron Ringstand Tongs Clamp Graduated Cylinder Test tube Unknown Metal Sample Hotplate Triple Beam Balance (or other mass measuring equipment) 600 ml Beaker Procedure: 1. Follow all safety guidelines prior to starting. Clear lab station. Gather all materials. 2. Set up the coffee-cup calorimeter as shown in the previous experiment in Figure 17-1. 3. Pour
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Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually iodine has a
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Chemistry Final Study Guide Matter as anything that has mass and takes up space. Significant figures refers to digits that were measured. When rounding calculated numbers‚ we pay attention to significant figures so we do not overstate the accuracy of our answers. Rules: -All nonzero digits are significant. -Zeroes between two significant figures are themselves significant. -Zeroes at the beginning of a number are never significant. -Zeroes at the end of a number are significant if a decimal
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assignment‚ you will dissolve a sample of NaCl in water and then measure the boiling point elevation for the solution. 1. Start Virtual ChemLab and select Boiling Point Elevation from the list of assignments. The lab will open in the Calorimetry laboratory with a calorimeter on the lab bench and a sample of sodium chloride (NaCl) on the balance. 2. Record the mass of the sodium chloride in the data table. If it is too small to read‚ click on the Balance area to zoom in‚ record the reading
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CHM1032L pre/post lab instructions Preparation is a key to success in this lab. For this reason‚ you are required to thoroughly read through the experiment information presented in the lab manual‚ and complete a pre-lab for each experiment you do. The prelab must be completed prior to the day of the experiment. Each Friday I will ask to see your completed prelab before I allow you to enter the lab. If you have not finished the pre-lab‚ I will not allow you to enter the lab and you will receive
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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Student’s Name Date of Experiment- 11/27/12 Date Report Submitted 11/27/12 Title: Caloric Content of Food Purpose: to be able to measure the energy content of foods Procedure: We are going to take food items and burn them to heat water to be able to determine the amount of “energy” a food source can emit. Data Tables: |Data Table 1: Food Item - Observations
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Materials: Brightly colored test tube rack‚ kim wipes‚ beaker for trash 1.5 ml microcentrifuge tubes Test plate Micropipetters and tips DI water Buffer solution … 4.5 to 8.8 I2Kl (grams iodine) Starch solution Enzyme (amylase) 80 degree Celsius water (HOT) Floating test rack Procedure: While controlling the amount of starch and the amount of buffer we use with a pH of 5.8‚ we want to investigate how changes in enzyme concentration affect reaction rates. First we put 500 ml of amylase from
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Materials: • Graduated cylinder • 2 marbles • Liquids to test such as water‚ corn syrup‚ canola oil‚ motor oil • Masking tape • 1 cm ruler • Stopwatch that is accurate to 0.1 or 0.01 seconds SAFETY NOTE: See Texas Science Safety Manual for lab and investigation guidelines: http://www.tenet.edu/teks/science/safety/safety_manual.html Engagement: Place a wooden ramp on a stack of books. Challenge students to predict which of three liquids (syrup‚ water‚ and motor oil) will reach the bottom
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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