The technique of gene cloning has important applications in medicine‚ biotechnology‚ agriculture and research. Discuss two applications of this technique‚ which in your opinion‚ have had significant impact on society. Gene cloning is achieved by inserting a required gene into a vector DNA. This produces Recombinant DNA and when introduced to a host cell the vector is copied. It divides to produce lots of copies of the gene of interest (Willey‚ Sherwood and Woolverton‚ 2012). There are many applications
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Difference between the Gene Regulation in Prokaryotes and Eukaryotes Gene regulation is important for all cells to be able to control gene repression and the amount of proteins produced by those genes. It helps to make sure the cell uses resources efficiently and express the right genes under the right conditions. Gene regulation can be found in both prokaryotic and eukaryotic cells. The methods of regulation differ between the two groups. Prokaryotic regulation mostly occurs by enzymes interacting
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However relative quantification of gene expression using qRT-PCR is highly influenced by the expression stability of internal control or reference genes used for transcript normalization of target genes. The use of inappropriate or unstable reference gene(s) can seriously impact the transcript quantification results leading to false inferences or misinterpretations21‚22. Accurate normalization is thus necessary for obtaining biologically meaningful expression data and hence qRT-PCR analysis greatly
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Gene Kelly and His Great Visual Stylings "When most people hear the name Gene Kelly‚ if they’ve even heard of him at all‚ they will likely think of the man who will forever be immortalized as that guy who sang and danced in the rain‚ but there is more to "that guy". Sure‚ he danced as he s plashed in puddles‚ but he also choreographed exactly how he would splash in them‚ he directed exactly how the camera would zoom in his face as he did so‚ and of course he had to act exactly as though he was
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Application of Molecular Techniques for Detection of Disease Resistant Genes in Tomato Breeding Lines for Guatemala Objectives: 1. Evaluate and modify methods for detection of Fusarium Race 2 resistance gene. 2. Evaluation of two step protocol for detection of Mi-1 gene. 3. Verification of marker for Ty-1 and evaluation for marker in Guatemala breeding lines. (This is a Geminivirus that is transmitted by the whitefly) 4. Use of molecular markers to detect geminivirus resistance source for Gu143
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Keith B. Kofoed Biology 1407 Horizontal Gene Transfer Responsible for Carotenoid Production in Aphids Horizontal Gene Transfer Horizontal gene transfer (referred to as HGT for the rest of the paper) is said to have occurred when an organism successfully incorporates genetic information from another organism into its own genetic makeup when the first organism is not the offspring of the other organism. HGT‚ or lateral gene transfer (LGT)‚ is used to describe both the artificial and natural
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Amajala et al Journal of Drug Delivery & Therapeutics; 2012‚ 2(4)‚ 85-89 85 Available online at http://jddtonline.info RESEARCH ARTICLE METHYLATION PROFILING OF TUMOR SUPPRESSOR GENES INVOLVED IN LUNG CANCER * Krishna Chaitanya Amajala‚ Vishwanath A‚ Kaladhar DSVGK and Bhaskar Reddy I Department of Bioinformatics & Biochemistry‚ GITAM Institute of Science‚ GITAM University‚ Visakhapatnam-530045‚ Andhra Pradesh‚ India *Corresponding author’s Mail: chy2ak@gmail.com Received
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"Gattaca"‚ written and directed by Andrew Niccol‚ depicts a futuristic world of a cold society‚ where one’s resume exists in their cells and genes. Although genes play a significant part to a successful life in this world‚ the path and success of one’s life is not determined solely on DNA as demonstrated by Eugene‚ Anton and in particular‚ Vincent who clearly "exceeds his potential". Vincent Freeman is cast into society’s redundant section as being an "invalid"‚ however he overcomes this burden
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and the assurance to a higher standard of future for their children. However‚ this may not be accurate for all families. One or both of the parents have chosen to work overseas because of the worsening economic condition in the Philippines. From genes to bequests‚ parents have important influences on the income‚ health and general living standards of their children as adults (Ermisch‚ 2003). Parents either working within the country or overseas influence their children in countless ways. They create
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The Selfish Gene Author: Richard Dawkins Book Criticism •“Genes cannot be selfish or unselfish‚ any more than atoms can be jealous‚ elephants abstract or biscuits teleological. This should not need mentioning‚ but Richard Dawkins’s book The Selfish Gene has succeeded in confusing a number of people about it” (Midgley). •There are many things to disagree with in the book‚ depending on one’s viewpoint. Some people may interpret the term “selfish gene” in a literal way‚ believing that
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