minutes at 72°C. The Amplified DNAs were analyzed by horizontal gel electrophoresis at 100 V in 1.5% agarose gel (wt/v) in 1X TAE buffer (0.04M Tris-Acetate‚ 0.001M EDTA‚ pH 8.0)‚ stained with 2.5X solution of GelRed™ Nucleic Acid Gel Stain (250μL GelRed™ to 1 liter of distilled water)‚ and gels were visualized under UV light and photographed. Afterwards the PCR amplified products were cut out from the gels and purified using the QIAquick Gel Extraction kit (Qiagen). Sequencing reactions were performed
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Carbohydrates are macromolecules whose basic units are called monosaccharides (or simple sugars). These simple sugars are polyhydroxy aldehydes (aldoses) or ketones (ketoses)‚ depending on the chemical nature of their carbonyl group‚ containing at least three carbon atoms. The smallest monosaccharides with only three carbon atoms are collectively known as trioses; while those with four‚ five‚ six‚ and so on carbon atoms are called tetroses‚ pentoses‚ hexoses and so on‚ respectively. Carbohydrates
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Layer Chromatography (TLC) is a solid-liquid technique in which the two phases are a solid‚ stationary phase and a liquid‚ mobile phase. The solid phase you will be using in today’s lab is a plastic plate covered with an adsorbent‚ in this case‚ silica gel. Aluminum is another common solid phase used. Because silica is a polar molecule‚ the components of the solution we will use in lab today will be separated based on their relative polarities. The more polar a molecule‚ the higher affinity it will have
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NcoI and XhoI (Fermentas‚ Lithuania) enzymes. The expression vector pET28a was also digested with NcoI and XhoI. The double digestion was carried out at 37ºC‚ digested fragments were analyzed by agarose gel electrophoresis and the products were purified (Fermentas‚ Extraction of DNA from the gel kit‚ Lithuania). The digested products‚ pET28a expression vector and V-Domain fragments‚ both were ligated by T4 DNA ligase‚ with the ration of 1:6 of vector and V-domain gene‚ and incubated first at 16 ºC
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So we’ve talked about what makes the DEWALT DG5204 so tough‚ but what makes them so comfortable? Firstly‚ the layered gel padding that surrounds the knee adds support and protection. The thick padding means they stay comfortable no matter what position you may find yourself in while working on flooring. The gel padding contours to your knee so you don’t get that feeling of having something digging into your knees‚ which is a common complaint among less well designed knee pads. The straps are a slip-buckle
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cream in grocery stores‚ but making it on your own can be really fun. There are only a few easy steps to make your own delicious ice cream. First‚ you will need to freeze the gel container from your ice cream maker. It usually takes about 6 to 22 hours depending on how cold your freezer is. To determine whether the gel container is completely frozen‚ just shake it. If you don’t hear liquid moving‚ it’s frozen. Next‚ you will need to heat the milk‚ sugar and powered milk. In a large pot (4 quarts
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it can be used for different surfaces so the researcher conducted an experiment. The researcher scraped the inner portion of banana peelings and boiled for four hours‚ extracted the Aloe Vera gel and blended to obtain a smooth gel. To perform the experiment‚ the researcher used 3:4 ratios of Aloe Vera gel and banana peelings then added 1:2 ratios of used cooking oil and gin. Nine people were asked to fill an evaluation paper about the product based from texture‚ luster‚ and odor it gives. All of
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water for about 15 minutes. This will help the Babyfoot gel penetrate the skin. The next step is to put on the Babyfoot booties. These plastic booties are lined with the Babyfoot gel. The kit comes with tape to tighten the booties down against the skin. Wearing socks can help the process. You will need to wear the booties about an hour. Just sit back and relax. Watch TV‚ read a book‚ browse the Internet. Then remove the booties and clean off the gel. That’s it. Your feet will look a bit dry for a couple
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It contains a highly cohesive gel that gives it a very natural feel. MemoryGel implants contain a proprietary cohesive gel that mimics the look and feel of natural breast tissue. Saline-filled implants come in all shapes‚ sizes‚ and profiles. They bring a slightly firmer feel than the gel implants. They are filled with saltwater very similar to what is already in the human body. Spectrum Saline implants offer
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Summary for Quiz Monday‚ May 30‚ 2005 12:13 AM Title Identification of Some Macromolecules Gist of Experiment • Use different tests to check for the existence of macromolecules in various substances o Iodine test checks for starch and/or glycogen o Benedict’s test checks for reducing sugars o Biuret test checks for protein Notes on Underlying Theory Introduction • The most abundant elements in living material are: o Carbon
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