Title: Cloning of Enhancer of Zeste Homolog 2 in forward orientation into Escherichia Coli using histidine-tagged pbluescript II KS+. Abstract Enhancer of Zeste Homolog 2 locus is intensely over expressed in breast and prostate cancer and it’s been established that its promoter inhibition by p53 has led to reduced cell proliferation and invasion (Bracken‚ 2003; Xiao‚ 2011). Objective is to clone a forward orientated EZH2 insert into a his-tagged pbluescript. Cloning EZH2 into a histidine-tagged
Premium DNA Polymerase chain reaction Molecular biology
* INTRODUCTION “Adequate inventories facilitates production activities and help to customers satisfaction by providing good service.” The basic financial aim of an enterprise is maximization of its value. At the same time‚ a large both theoretical and practical meaning has the research for determinants increasing the firm value. Most financial literature contains information about numerous factors influencing the value. Among those factors is the net working capital and
Premium Inventory
For your Written Assignment‚ begin by reviewing the ABA model rules. Next research what role ethics have played in the development of American law and how ethics are used every day by lawyers and paralegals. The following questions should be addressed in your paper. Why are ethics so important in American Law and how do they help shape the laws in this country? Explain three important ABA Model Rules along with examples that apply to law firms. Explain three important ABA Model Rules along
Premium Ethics Philosophy Morality
3.2.4 Genomic DNA extraction of the root tips After the treatment of the onion bulbs with lake water sample at different period of time‚ the DNA from the root tips will be ready to be extracted and observed. First of all‚ the genomic of the Allium cepa will be easily done by the already available Uneasy® Plant Mini kit (Qiagen‚ Germany) in the laboratory. All of the procedures can be performed by referring to the manual provided by the kit. A very small amount of root tips that weigh 100 mg will
Premium DNA Bacteria Gene
lipophilic character‚ and slow dissolution in aqueous solutions. As a result‚ oral drug administration leads to occurrence of neurological reactions and gastrointestinal disorders. It is a productive drug when used topically in the form of creams‚ gels‚ vaginal tablets‚ suppositories lozenges‚ and ointments. However‚ its low solubility is a complication in the treatment of cutaneous diseases through topical delivery. The drug should be administered to the site of infection in a topical dosage form
Premium Candidiasis Candidiasis Fungus
temperature at which the thermostable DNA polymerase in the mix will operate most efficiently in synthesizing new DNA strands. Gel Electrophoresis exposes the molecular sizes of different DNA fragments as the lightest or shortest fragments travel fastest down the gel and the heaviest or largest fragments travel most slowly and are left near the top part of the gel. In this run‚ samples A-F show almost identical bands‚ indicating that all six samples are the same DNA. Two bands are found in each
Premium DNA Molecular biology Gene
The Benefits Of Aloe Vera For Beauty As for the benefits of Aloe Vera are as follows Since Aloe Vera medicine plant once known as efficacious. Although a regular in the pot is grown as an ornamental plant‚ but Aloe Vera is often used for beauty treatments‚ from start to nourish hair to get rid of acne. Aloe Vera contains a variety of biological active compounds‚ such as acetic mannans‚ polymannans‚ antrakuinon‚ variety of lectin and saponins and compounds antrakuinon. Antrakuinon compounds found
Premium Skin
____________________ ABSTRACT The effect of molecular weight on the diffusion of substances were tested through the use of agar-water gel with three wells placed in a petri dish. Drops of three different substances namely: Potassium permanganate (KMnO4)‚ Potassium dichromate (K2Cr2O7) and Methylene Blue were placed in the wells of the agar-water gel in the petri dish. The diameter of the diffused substance was noted every 3 minutes for 30 minutes. The Potassium permanganate (KMnO4)‚ which
Free Diffusion Molecular diffusion Ammonia
person needs to complete the cleanse. It includes the meal replacement drinks‚ fiber packets‚ supplements containing contain garcinia cambogia extract and green tea extract and Forever Aloe Vera Gel. People on the Clean 9 program drink 1/2 a cup of the Forever Aloe Vera Gel daily with a glass of water. The gel contains water‚ soluble fiber and nutrients and it promotes digestive health. A shaker‚ tape measure and exercise
Premium Obesity Nutrition Weight loss
Analysis of A. fischeri chDNA restriction digestion by Sal I enzyme after isolation and purification via agarose gel electrophoresis Bio 219-064 (Techniques in Molecular Biology) Group 5: Tung Nguyen‚ Uyen Tran‚ Amber Beckley‚ Danielle Exler Department of Biology‚ Drexel University‚ Philadelphia‚ Pennsylvania 19104 Submitted October 27‚ 2014 ________________________________________________________________________ Abstract Bioluminescence is one of the benefits that a deep sea organism received
Premium Molecular biology DNA Polymerase chain reaction