by the CFCs or HCFCs. Increasing recognition of environmental problems associated with CFCs and HCFCs has opened favourable opportunities for the development of green air conditioning technologies. This project report presents a revolutionary silica gel – water adsorption system for air conditioning in automobiles. The cooling effect is achieved by recovering waste thermal energy from the exhaust gases. The system is cheap and easy to fabricate. The refrigerant‚ being water‚ is environment friendly
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Introduction: Escherichia coli (E.coli) is a commensal-pathogenic organism which include a wide range of strains. There are several advanced molecular-genomic technologies that are capable for detecting and identifying different strains of E.coli. But‚ ERIC-PCR technique is a quick and cost effective method for determining individual strains via demonstration of strain specific fingerprint bands. Therefore‚ the ERIC-PCR technique was used to determine the isolated strains of E.coli from different
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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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Buffer (50 X) (2 M Tris‚ 50 mM EDTA) 2L 484 g Tris 114.2 ml glacial acetic acid 200 ml 0.5 M EDTA 8.0 To make 1x TAE 20 L‚ add 400 ml 50X buffer into 19.6 L ddH2O. 4. SDS-PAGE Gel Solutions Vol (L) Tris (g) HCl (ml) 10% SDS (ml) 4x Lower gel buffer 1.5 M Tris-Cl‚ pH 8.8‚ 0.4% SDS 2 363.3 50-60 80 ml 4x Upper gel buffer 0.5 M Tris-Cl‚ pH 6.8‚ 0.4% SDS 2 121.1 70-80 80 ml 4.1 10% SDS 1L: 100g SDS into 1 L‚ heat to 68oC for solubility. pH ~6.6. 5. 5X SDS Loading Sample Buffer 100 ml
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Analysis of the RBM22 Protein Association with Gene Expression through Affinity Tagging Lucas Akin Stream Faculty: Scott Stevens Research Professor: Albert MacKrell University of Texas at Austin School of Biological Sciences Abstract Affinity tagging of proteins is an important method for biochemical analyses [4] and has proven effective in further expanding our knowledge of their specific functions. A group of interactive proteins expressed by bacteriophage ʎ known as exo‚ bet‚ and
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Goal: 9 Myths About Teeth Whitening and Why You Should Ignore Them! Word count in this document: 800 Title: 9 Myths About Teeth Whitening and Why You Should Ignore Them! Do you feel as though it’s time to get your teeth whitened? Most of us want to get them whitened at some point or another. Maybe it’s because of stains on your teeth‚ or maybe it’s because you just want a fresh‚ new look. Whatever the reason is‚ it’s important to get your teeth whitened at the dentist‚ and to not do it by yourself
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Restriction Fragment Length Polymorphism and Southern Blotting 1. Abstract The aim of the experiment was to be introduced to the techniques involved in the identification of restriction fragment length polymorphisms. Restrictions were carried out using three different restriction enzymes‚ ECORI‚ HindIII and BstELI with their buffers. Lambda (λ) DNA was then examined using electrophoresis and Southern blotting. The results showed that λ DNA was best digested by EcoRI as all of the expected bands
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in a modern shaving preparation. SHAVING GEL Shaving gel is a lubricating product designed to be applied before shaving in order to soften the hair being shaved‚ along with moisturizing the skin and preventing the razor from dragging along the skin‚ potentially creating razor burn. It comes in tubs as well as cans‚ and is usually designed to foam into a thick‚ dense lather. Most companies which manufacture conventional shaving cream also make shaving gel‚ as many of the basic ingredients are the
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SUMMARY Skin-Tique Corporation manufactures women’s personal care products. The products consist of; facial creams‚ hand and body lotions‚ and a full line of women’s toiletries. Soft and Silky was positioned as a high-quality women’s shaving gel since its introduction in 1988. Phoebe Masters‚ the newly appointed Product Manager for hand and body lotions at Skin-Tique‚ recognized that they face some problems. Also Heather Court right‚ the Soft and Silky Brand assistant suggested them it is time for
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used to soothe or get rid of the pimple very quickly. Natural Treatments: Aloe Vera: The liquid extract or gel from Aloe-Vera plant is excellent for soothing individual pimples and acne as well as helping to prevent a breakout. The skin absorbs this gel 4x’s faster than water‚ plus the ingredients in the gel soothe the irritated pores. The excellent anti-bacterial properties of the gel reduces the inflammation by quickly penetrating through several layers of tissues and breaking up residue‚ dead
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