iIsolation of Chlorophylls and Beta Carotene from Plant Leaves Wang Haina A0133901R 1. Aim 1. To isolate chlorophyll and beta carotene from plant leaves using column chromatography. 2. To qualitatively characterise the pigments with UV-vis spectroscopy and TLC. 2. Results and discussion Isolation of beta carotene and chlorophylls by column chromatography Upon the loading of S1 (the extract of the plant leaves in hexane)‚ a yellow band appeared at the top part of the silica column immediately
Premium Chlorophyll Chromatography Thin layer chromatography
-Tique Corporation must decide if and how the company will introduce an aerosol can package for its Soft and Silky Shaving Gel‚ including a determination of product size (5.5 or 10 oz.) and location (personal care or toiletries). Furthermore‚ the manager needs to decide if a test market is necessary to evaluate the new packaging. Strategic Issues Product Soft and Silky Shaving Gel (SSSG)‚ a women’s shaving product that contains moisturizers‚ is currently packaged in a 5.5 oz. plastic tube and is located
Premium Marketing Product management Revenue
Colon Cancer Scenario Introduction: Gel electrophoresis is an important molecular biology tool: it enables us to study DNA. It can be used to determine the sequence of nitrogen bases‚ the size of an insertion or deletion‚ or the presence of a point mutation; it can also be used to distinguish between variable sized alleles at a single locus and to assess the quality and quantity of DNA present in a sample. Gel electrophoresis is a method of separating chemical compounds and molecules by their size
Premium Molecular biology DNA Gel electrophoresis
The picture above shows a typical gel electrophoresis set up. The clear container in the center of the picture is called a gel electrophoresis chamber. It contains the agarose gel that will be loaded with genetic material‚ as well as a buffer solution. It is connected to a DC power supply via electrodes. This picture was taken at Paw Print Genetics laboratory in Spokane‚ Washington. Viney and Fenton (1998) defined the term electrophoresis as‚ “the migration of charged particles through a static medium
Premium Electric charge Electromagnetism Electron
DNA Isolation and Visualisation The laboratory work today consists of three stages. 1. Separation and visualisation of DNA on agarose gel 2. DNA extraction 3. Virtual electrophoresis Part 1 involves casting a gel‚ which then requires approximately 30 minutes to set‚ followed by 1 hour to run. Consequently‚ you might want to cast the gel and let it set whilst you undertake Part 2 - your DNA extraction. Make
Premium DNA Molecular biology Polymerase chain reaction
lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin‚ and carbonic anhydrase) will be placed into separate wells within the gel‚ and the apparatus will be connected
Premium Gel electrophoresis Molecular biology DNA
Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results 1 2 3 4 5
Premium Molecular biology Gel electrophoresis DNA
Making an Agarose Gel AIM: To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also‚ to extract DNA from wheat germ. INTRODUCTION: Agarose gel is a substance that is used in science for gel electrophoresis and size exclusion chromatography. These processes use agarose gel to separate and analyze proteins and DNA. The medium is composed of a purified agarose powder that has been boiled in a buffer solution and then cooled into a gel. Agarose gel is most commonly
Premium DNA Molecular biology Gel electrophoresis
piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells‚ then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain restriction enxymes and separated using gel electrophoresis‚ then the smaller the DNA fragment cut‚ the greater the distance it will travel in the gel. Variables: The control
Premium Bacteria DNA Molecular biology
the prevalence of the Borrelia‚ Rickettsia and Ehrlichia species (the causes of the diseases mentioned above) in the tick A. inornatum‚ the Polymerase Chain Reaction (PCR) and Agarose Gel Electrophoresis procedures are applied. The PCR method is used to amplify the DNA samples of the tick studied‚ while the Agarose Gel Electrophoresis method is run in order to identify whether the species of bacteria of our interest are present in the DNA samples or not. The results obtained‚ so far‚ show 23.5% positive
Premium Lyme disease Gel electrophoresis Polymerase chain reaction