restrict the discussion to a particular area‚ eg in ‘Chapters 1-3’‚ during the ‘nineteenth century’. Finish any necessary reading or research as background to the essay Be selective: use sources which are relevant and accessible. Write notes in your own words. Write down quotations that may be particularly useful‚ but ensure the source of these quotes is acknowledged if they’re used. Take note of sources so they can be provided in footnotes and the bibliography. Brainstorm ideas in response to
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PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
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Light Microscopy Lab Lab write-up 1 Parveen Rajper Biology 101 Principles of Biology 1 September 29‚ 2012 Introduction: The light microscope helps see the microscopic world that the naked eye cannot see. If there were no microscopes in the world‚ than it would be very difficult to understand the many particles that surround the living world. The purpose of this lab experiment is to become familiar with a microscope. It is hypothesized that it will be difficult
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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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How to write an abstract An abstract is a short summary of your completed research. If done well‚ it makes the reader want to learn more about your research. I .These are the basic components of an abstract in any discipline: 1) Motivation/problem statement: Why do we care about the problem? What practical‚ scientific‚ theoretical or artistic gap is your research filling? ( Some state the background information about the investigation briefly before the motivation) 2) Methods/procedure/approach
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carbohydrates. Procedure Preparing the lab supplement: First‚ canned spinach supplied by the organic chemistry laboratory in room
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California. Laboratory agar looks gelatinous because it is dissolved in boiling water and cooled. Agar can be easily distinguished from the other materials commonly found in a laboratory due to its distinctive smell. At room temperature‚ agar is a gel and it stays firm at 65ºC and melts at 85ºC. It solidifies when the temperature reaches 32 – 40ºC. It is used as various microoragnisms’ culture medium‚ especially for bacteria and it is also served as a thickening for soups and sauces‚ in jellies and
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Solve the Murder Koreen Clarke October 29‚ 2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette
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Writing an essay Define the context of your essay. The context is the scope of the essay. What are you being asked to write about? The context can include multiple parameters including: Topic. Sometimes your teacher or professor will give you a prompt that you’re required to use. If you do get an option to pick your own topic‚ then choose something that you genuinely want to become an expert about or something you feel passionate about discussing. Format. The length of the essay‚ the way the
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room temperature and heated using a rotational viscometer which has shear rates of 600rpm; 300rpm‚ 200rpm‚ 100rpm‚ 6rpm‚ 3rpm or “gel” and 10second and 10minute gel can also be found. These rate especially the 600rpm and the 300rpm are then used to calculate PV (deals with the total solids) and YP (deals with the carrying capacity of the mud)‚ the gel is related to the gel strength which deals with the suspended capacity. From analysis when heated as compared to when at room temperature the PV and
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