DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation‚ scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites‚ ensuring that all DNA fragments that contain a particular sequence have the
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subject: Serum protein electrophoresis. Report No.: 1. Serum Protein Electrophoresis (SPEP) Introduction: The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to help identify some diseases. And its uses an electrical field to separate the proteins in the blood serum into groups of similar size‚ shape‚ and charge. And here we’ll use gel electrophoresis which indicate that blood serum is placed on special paper treated with agarose gel and exposed to an electric
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Marissa Arias Senior Rhetoric Graf-8 April 14‚ 2014 Gel II Manicure Many women and even some men love to get their nails done‚ but hate how much time it takes to dry and on top of that they are extremely easy to ruin and chip. There is a new type of manicure trending know as Shellac‚ Gel nails‚ or No chip. According to my cosmetology instructor Samantha Rudman‚ this process takes about 35 to 40 minutes but dries in seconds. Many individuals would love to know this process even the men that
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Agarose Gel Electophoresis of DNA Topoisomers Introduction DNA can exist as different isomers that change the confirmation of the DNA’s structure. DNA can be in a linear confirmation this is a relaxed confirmation as the DNA can rotate about its axis unconstrained. It can also exist as a nicked circle this is also a relaxed confirmation as the DNA strands can again rotate freely with respect to one another. Covalently closed circular DNA or cccDNA exists as a supercoil this is because the covalent
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Bio 205 Lab W/8:00 Enzyme II Write-Up Methods: My partner and I ran two experiments to measure the activity of the enzyme horseradish peroxidase under varied conditions. The first of which measured the effects of altered pH levels‚ while the goal of the second was to examine the effects of varied temperatures. To test the effects of pH on horseradish peroxidase‚ we began by zeroing a Spec 20 with 5.0mL of substrate (25mM guiacol) at pH 6.5. Once the Spec 20 was accurately zeroed‚ we added 100μL
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Littlefield Simulation Write-up December 7‚ 2011 Operations Management 502 Team 9 Littlefield Lab We began our analysis by searching for bottlenecks that existed in the current system. It was easily identified that major issues existed in the ordering process. Without calculations‚ you could tell the reorder point was too low since the historical plots showed inventory levels at zero for two or more days at a time. The number of jobs in customer orders showed correlating spikes at the same
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Electrophoresis of Amino Acids Introduction: Electrophoresis is a separation technique based on the movement of charged ions under the influence of an electrical field. This technique is primarily used for the separation of amino acids and peptides on the basis of their charge. All amino acids contain ionizable groups that cause the amino acids‚ in solution‚ to act as charged polyelectrolytes that can migrate in an electric field. The amino acids with a net positive charge will migrate toward
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on the filter paper. Caution should be used as the root tips are very fragile 5. Transfer one of the garlic root tips to the clean microscope slide. Cut about 2-3 mm from the rounded growing tip. Keep this growing tip and discard the rest. 6. Break up the root tip with the mounted needle (this is called maceration). Add one small drop of toluidine blue and leave to stain for 2 minutes 7. Cover with the cover slip and blot firmly with several layers of filter/ tissue paper and press gently to spread
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BSC 1005 M-F 12:00 – 1:00 LAB 1- SCIENTIFIC METHOD HOW FAR CAN A MEALWORM CRAWL? Observations: 1. The scientific method allows for an organized and systematic approach to problem solving. It represents the “backbone” of scientific research‚ as well as a useful method in everyday decision making. The steps involved in the scientific method would include‚ but not be limited to‚ the following: 1. Statement of problem 2. Information gathering on research 3. Formulation of hypothesis/possible
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Agnes Wong Professor Coyle Auditing-Case Write Up 30 September 2014 The Leslie Fay Companies Case Summary The Leslie Fay Companies is a women’s apparel manufacturer headquartered in New York‚ but with its accounting offices located in Pennsylvania. The company performed business in a way that did not utilize modern computerized systems to track sales and growth‚ but in an old-fashioned way that yet‚ still let them perform well in their revenues and earnings. The major names in this case include
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