Introduction During the course of this lab‚ we explored whether or not certain processed foods contained Genetically Modified Organisms. Genetically modified food is an important subject in the world today. Ever since Flavr Savr came out with their tomatoes grown from genetically modified seeds in 1994 genetically modified foods has become ever more popular amongst distributors of produce (Mestel‚ 2013). The world’s population has grown by around one billion in the last decade (US Bureau of the
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DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation‚ scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites‚ ensuring that all DNA fragments that contain a particular sequence have the
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CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8.8 and stacking gel‚ prepared using
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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Presence of Alu element within PV92 on chromosome 18 in a mixed ethnical population and its connection with humans’ ancestral origins By Shiyu Song Biology 155Q LAB-A: 14:30-17:30 Dr. Jacob April 17th 2013 Abstract The Alu insertion (+) within PV92 on chromosome 16 usually reflects a person’s ancestral origin. This study aimed to find the correlation between the presences of Alu insertion (+) within PV92 on chromosome 16 within a population. We used PCR to detect the Alu insertions
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Marissa Arias Senior Rhetoric Graf-8 April 14‚ 2014 Gel II Manicure Many women and even some men love to get their nails done‚ but hate how much time it takes to dry and on top of that they are extremely easy to ruin and chip. There is a new type of manicure trending know as Shellac‚ Gel nails‚ or No chip. According to my cosmetology instructor Samantha Rudman‚ this process takes about 35 to 40 minutes but dries in seconds. Many individuals would love to know this process even the men that
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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Agarose Gel Electophoresis of DNA Topoisomers Introduction DNA can exist as different isomers that change the confirmation of the DNA’s structure. DNA can be in a linear confirmation this is a relaxed confirmation as the DNA can rotate about its axis unconstrained. It can also exist as a nicked circle this is also a relaxed confirmation as the DNA strands can again rotate freely with respect to one another. Covalently closed circular DNA or cccDNA exists as a supercoil this is because the covalent
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Now You See It – Copper Cycle Lab The purpose of the lab is to discover what happens when someone executes a series of procedures‚ beginning with copper metal. What is done | What is observed | 1. Started with copper‚ Cu (s). | reddish‚ brownish‚ orange-ish‚ powder-like | 2. Added nitric acid‚ HNO3 (aq). | acid turns blue and smells like chlorine. | 3. Added water‚ H2O (l). | stayed the same | 4. Added sodium hydroxide‚ NaOH (aq). | changed consistency‚ gel-like | 5. Heated the mixture
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Electrophoresis of Amino Acids Introduction: Electrophoresis is a separation technique based on the movement of charged ions under the influence of an electrical field. This technique is primarily used for the separation of amino acids and peptides on the basis of their charge. All amino acids contain ionizable groups that cause the amino acids‚ in solution‚ to act as charged polyelectrolytes that can migrate in an electric field. The amino acids with a net positive charge will migrate toward
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