Lab 5 Column Chromatography: Isolation of Lycopene from Tomato Paste Reading: Zubrick‚ pages 79-82‚ 127-130‚ 138-139‚ 141-143‚ and 235-240 Pre-lab: look up the structure of lycopene. Introduction: Lycopene is the red pigment in ripe tomatoes and‚ as an antioxidant‚ helps to fight certain cancers. In this lab you will isolate lycopene from tomato paste. To do this you will first extract carotenoid pigments from the paste and then use column chromatography to isolate the lycopene from
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Column and thin layer chromatography of plant pigments CHEMISTRY 201L EXPT 04 PAGE 01 - 12 Noel Angelo P. Kalacas*‚ Hanna Mae Laluces‚ Ina Bianca Lanuza Department of Chemistry‚ College of Science *Corresponding author; e-mail: knight_BeNcH66@yahoo.com Abstract Chromatography is a powerful technique for separating and/or identifying the components in a mixture. There are different types of chromatography and each has its own strengths and weaknesses. In this experiment‚ pigments of the
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The Politics of Natural Gas in Bangladesh: An Attempt to Explicate the Kleptomaniac Nexus between Western Imperialism and Local Comprador Bourgeoisie COURSE NAME & NO.: Understanding Social Change in Bangladesh‚ 481 TO SUBMITTED BY Dr. A I Mahbub Uddin Ahmed MD. Nazmul Arefin Professor‚ SUBMITTED
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1.5.2.5 Detectors The detectors used in UPLC should be able to handle very fast scanning methods because half-height peak widths of less than one second are usually obtained with columns packed with 1.7 µm particles. The detector must be able to give high sampling rate adequate to capture enough data points across the peak for an accurate and reproducible integration of analyte peak. The dispersion (volume) of the detector flow cell must be minimal to maintain separation efficiency. Conceptually
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Purpose: Unknown mixtures will be separated by means of chromatography in which the mixture will be passed in a solution through a medium leaving behind components of the mixture at different rates‚ therefore‚ different spots on the absorbing substance. This will help determine the identity of unknown mixtures. The spot colors on the strip of filter paper and the Rf values of the unknown samples will be compared to those of known samples. To find the position of the spots on the strip of paper‚ we
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Yuri Anthony D. Sucgang BMLS-IA Electrophoresis Machine Gel Electrophoresis Apparatus(Aragose Gel) Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. Gel electrophoresis is a laboratory procedure used to separate biological molecules with an electrical current. In this lesson‚ we’ll review how agarose gel electrophoresis works and introduce the equipment necessary to perform an electrophoresis experiment.
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Ion Exchange Chromatography Week 10 TAG Question 1: Chapter 6-4 of your technique book provides a detailed description of how to run a ion- exchange column. Assume you have a cation-exchange column already prepared and ready to use. Create an outline of no more than 10 steps describing how you will regenerate the column‚ load your sample and collect the hydronium ion released. 1. Open the stopcock at the bottom of the column and allow solution to drain out until the solution level in the
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A simple‚ rapid‚ specific and highly sensitive spectrofluorimetric method has been developed for the quantification of dabigatran etexilate mesylate (DAB) in bulk and capsule dosage form. A linear relationship was found between fluorescence intensity and DAB concentration in the range of 0.01-1.0 μg/ml in DMSO as solvent at an emission wavelength of 391 nm after excitation at 334 nm‚ with a good correlation coefficient (0.989). The detection and quantification limits were found to be 0.005 and 0
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Name _____________________________ Date ____________ Period _______ Completing the Square Day 1 For each expression‚ find the number you would add to make it a perfect square trinomial. Leave fraction answers as improper fractions (no mixed numbers or decimals). Then factor each trinomial. 1. x2 + 10x + _______ 2. y2 – 6y + ¬¬¬¬_______ 3. z2 – 8z + _______ _____________________ _____________________ _____________________ 4. x2 + 12x + _______ 5. x2 – x + _______ 6.
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PAPER AND THIN-LAYER CHROMATOGRAPHY Lab 47 Michael Stravino‚ CHM 2211L‚ June 3‚ 2012 Abstract Thin-Layer Chromatography and Paper Chromatography can be used to identify identical compounds‚ unknown compounds‚ the number of components in a mixture‚ as well as other important uses not explored in this laboratory. This experiment explores these techniques to identify an unknown amino acid by TLC and analyze food colors using paper chromatography. By using 2.0 mL of dimethoxyethane and 18 mL of
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