Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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distillation and gas chromatography based on their difference in boiling points. The boiling point of hexane is 69 degrees Celsius and the boiling point of toluene is 110 degrees Celsius. Three fractions were collected for both simple and fractional distillation. The first fraction was hexane since it had the lower boiling point. The second fraction was a mixture of toluene and hexane. The third fraction was toluene since it had the high boiling point of 110 degrees Celsius. Gas chromatography was also preformed
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Lab: Gas Laws Purpose: Obtain a reference of temperatures effect on gas using Charles’ law when heating a capillary tube in water on a heated hot plate. Then‚ cooling the same capillary tube with ice while measuring the temperatures cooling effect on the gas bubble inside the capillary tube. Measurements of temperature change are taken with microLAB sensor and graphed using microLAB software. A final determination of experiments determined absolute zero versus actual absolute zero will be
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This report will cover the adaptations relating to gas exchange in insects‚ fish and mammals. These all have different gas exchange systems but work in similar ways. All the animals studied have the same characteristics in order for them to be able to work properly. Overview The gas exchange system is the process in which oxygen enters the cells of many living animals. Some factors that affect how efficient a gas exchange system is are things such as the distance that the molecules have to go before
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Separation of Amino Acids by Cation Exchange Chromatography Introduction and Purpose: Amino acids are small biomolecules that have a carboxylic acid backbone in common‚ as well as an amino group attached to a saturated carbon. There are many amino acids‚ but there are 20 most commonly know amino acids. Amino acids are the fundamenta building blocks of other biomolecules like proteins and ezymes (Davidson‚ 2015). This experiment examined a mixture of 3 amino acids. The purpose of this experiment
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possible to identify which peak correlates with which compound. Figure 2 contains a peak at around 500 and 700 nm which is a good indicator that that would-be chlorophyll b. This makes sense because this was the bottom band from the thin layer chromatography and chlorophyll b is the most polar compound. Chlorophyll B contains an aldehyde where chlorophyll A has a methyl group making it slightly more polar. Following that‚ figure 4 contains
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Task 3: How could Thin Layer Chromatography (TLC) have been used to prepare a pure sample of aspirin? The method of using thin layer chromatography is easy‚ quick and relatively cheap to carry out this makes it ideal for preparing pure samples of aspirin. Equipment Required: • Thin Layer Chromatography Paper • Ruler • Pencil • Solvent • Beaker • Micro Capillary • Access to a fume cupboard • UV light • Clingfilm • Individuals sample of aspirin • Pure sample of aspirin • Sample of salicylic acid Method:
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any one factor that affects retention on paper chromatography Design and carry out a scientific investigation on any one factor that affects retention on paper chromatography CHEMISTRY LAB REPORT CHEMISTRY LAB REPORT Name: Sri Danielle Class: 9A Teacher: Sir Ajay Name: Sri Danielle Class: 9A Teacher: Sir Ajay Aim My aim for this experiment is to find out how different types of solvent will affect the retention factor in paper chromatography. Hypothesis I think that there will be a
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S5E1 Gaseous Diffusion 1.0 ABSTRACT In this experiment‚ our objective is to find out the gas diffusion coefficient‚ D of acetone in the air. This experiment is conducted at a temperature of 50ºC and atmospheric pressure. The method that is applied to conduct this experiment is called the Winkleman method where the level of acetone (Z) is determined every 15 minutes by using a microscope. With the level of acetone being determined‚ a graph of t/L+Lo (min /mm) vs. L-Lo (mm) is plotted and the
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Is Higher Education Worth the Price? The question of whether or not higher education is actually “worth it” is an entirely subjective on. To objectively determine if higher education will give a high return on investment‚ one needs to first understand what they are hoping to gain out of the entire experience. Higher education does worth the price because it helps students to build their self-fulfillment‚ provide more monetary opportunities and reach their final goal . In the United States‚ for
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