is how to identify unknown specimens. • Why is this important? • Labs can grow‚ isolate and identify most routinely encountered bacteria within 48 hrs of sampling. • The methods microbiologist use fall into three categories: ♣Phenotypic- morphology (micro and macroscopic) ♣Immunological- serological analysis ♣Genotypic- genetic techniques Dr.T.V.Rao MD 2 Microbe Identification • The successful identification of microbe depends on: ♥ Using the proper aseptic techniques. ♥ Correctly
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skin then transferred using the aseptic transfer technique into the sterile media. Incubated both specimens for 48 hours observed and recording results of growth at 24 and 48 hours. After observing final growth pattern at 48 hours prepared both wet mount and direct stain slide for each of the cultures. Viewed under microscope using both the 40X and 100X oil immersion lens. Disinfected work area. Observations: |Bacteria |Growth pattern after 24 hours
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structures of the cell‚ which would otherwise be unseen. Further‚ in order to use the oil immersion objective of the microscope and thereby obtain the greatest degree of magnification‚ it is convenient to use stained preparations rather than wet mounts.1 Although bacteria do not appear greatly different from their
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Chapter II Review of Related Literature Chemical Wood Pulping Chemical wood pulping involves the extraction of cellulose from wood by dissolving the lignin that binds the cellulose fibers together. The 4 processes principally used in chemical pulping are kraft‚ sulfite‚ neutral sulfite semichemical (NSSC)‚ and soda. The first 3 display the greatest potential for causing air pollution. The kraft process alone accounts for over 80 percent of the chemical pulp produced in the United States
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directly underneath the microscope but putting a drop of water on the specimen can improve how the structures appear under microscope and also prevent the specimen from drying out on the slide (2). Adding water to the specimen is called wet mount. The liquid used in wet mount fills the space between the slides to support the specimen and also allows light to pass through easily (3). The liquid is usually water can also
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recombination. Much of this evidence has come from studies done at the Evolution Canyons in Israel. There are four “Evolution Canyons”‚ each of which consists of two mountain slopes with varying climates. Evolution Canyon I‚ located in Lower Nahal Oren‚ Mount Carmel‚ contains a south facing slope (SFS) which experiences harsh climatic conditions‚ such as high temperatures and drought. The opposing north facing slope (NFS) is characterized by mild climatic conditions‚ including cooler temperatures and higher
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D. Technical Aspects a. Production program 1. Products: hot and cold coffee‚ coffee blends‚ cakes and pastries 2. By-products: 1000kg of fresh berry gives about 400kg of wet waste pulp and only 160kg of exportable green bean (Source: Jan von Enden - An Introduction to Good Manufacturing Practices for Post Harvest Processing of Arabica Coffee in Vietnam). Coffee pulp is mainly composed of water and sugar. The sugar can be fermented by bacteria and acidified‚ resulting in a bad smell. The water
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carmine on a wet mount slide‚ and by using dialysis tubing‚ we investigate the selective permeability to reducing sugar‚ glucose‚ starch‚ and iodine potassium iodine. In experiment 5.2 we use samples of ox blood and Elodea plant to observe the net flow of water from the surrounding solution into the cells. In experiment 5.3 in order to estimate the osmolarity of the potato tuber cells we use the methods of change in weight and change in volume. In experiment 5.1 while observing the wet mount‚ the Brownian
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seventy to ninety percent of the total yeast population (Ardhana). As previously stated‚ Kloeckera apis is a yeast or yeast-like organism with the full classification as follows: Fungi‚ Ascomycota‚ Saccharomycotina‚ Saccharomycetes‚ Saccharomycetidae‚ Saccharomycetales‚ Saccharomycodaceae‚ Kloeckera (Smith). The morphology of the colonies of Kloeckera apis exhibit an irregular shape‚ low convex elevation‚ and undulate edges. They are beige in color with an opaque texture. Individual cells of Kloeckera
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present. I am doing his experiment to verify the concept that microorganisms are everywhere and I most certainly expect my results to prove so. I expect to find many shapes‚ sizes‚ colours and indeed types of microorganisms present when I observe the morphology of the colonies present. Materials and methods: Swabs. Diluent 0.1% peptone. TSA (Trypticase
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