Objective: DNA is analyzed by agarose gel electrophoresis after being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then‚ EcoRI buffer‚ EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used to
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PROCEDURES IN IMPORT & EXPORT DOCUMENTATION |SL. NO. |SUBJECT |PAGE NO. | |1 |Significance of Documentation | | |2 |Shipments through ICD | | |3 |Customs procedures
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Big Business Starts as Small Business Software to support a growing enterprise There are two certainties in life – death and taxes. While both are unavoidable‚ at least the taxes issue can be managed. But managing taxes‚ and business finances in general‚ takes detailed information. Considering how most small businesses get their start in accounting for their business operations‚ it is not surprising that information gathering becomes one of the most time -consuming and frustrating tasks around
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University of Manchester University of Manchester NISHANT AGARWAL The report primarily discusses results obtained from the conducted experiments and includes computed data from the Kingston computer program. The value of the plastic moment capacity of each experiment was obtained; Obtained value was used to find the yield strength of the structures and compare them to typical values for mild steel. The important data is summarized in a table; followed by a list of important formulae. All
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Objective The objective of this experiment is to compare the theoretical internal moment with the measured bending moment for a beam under various loads. Introduction and Background Theory Definition of a Beam Members that are slender and support loadings that are applied perpendicular to their longitudinal axis are called beams. Beams are important structural and mechanical elements in engineering. Beams are in general‚ long straight bars having a constant cross-sectional area‚ often classified as
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FLEXURAL BEHAVIOUR OF POTASSIUM BASED GEOPOLYMER CONCRETE. D.Vasuki1‚ A.Kalaivani2 1PG Student‚ Department of Civil Engineering – Paavai Engineering College. 2Assistant. Professor‚ Department of Civil Engineering – Paavai Engineering College. ABSTRACT The global usage of cement occupies the second position next to water. As the development of country and infrastructure‚ their materials needed get increased. Meanwhile for the development of the infrastructure concrete plays the
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total ) x 100 Major component: superstructure x2‚ loading component‚ pin holder‚ flexor‚ spring‚ pin No. functional component = 6 Total no. component = 9 Design efficiency= (6/9)x100 = 66.67% ii) Suitability A breakdown of how the unit is currently manufactured and associated costs a) Sequence of assembly: 1. Flexor + top superstructure + PVC holder 2. Bottom superstructure + PVC holder 3. Loading compartment + Pin holder + Spring 4. Step 1 + Step
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Pirated by Ulitos‚ YAAAAARR! ******************************************* __ ___ ______ ______ / / / / / __ \ / _ \ / / / / / / |_| / / | | / // / | |___ / /_/ / / / \___ \ / ____ / / /| | ___ \ | / / / / | | \ \_/
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this experiment‚ the best choice of buffer to use is TAE. The result of our experiment showed no bands. The reasons can be whether the concentration is either very low or DNA is not present at all. This may occur if students punctured the well while loading their DNA. _____________________________________________________________________________ Results and Discussion Gel electrophoresis is a method for separation and analysis of macromolecules (DNA‚ RNA and proteins) and their fragments‚ based
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Changing Attitude of Indian Customer’s towards Credit Cards *Dr. Saraju Prasad Abstract: In recent years‚ economic pressures‚ heightened competition‚ rapid technological developments‚ and a decline in consumer purchasing power are paving the way for the change in global market for the credit card business. The aforementioned changes and transformations are also affecting credit card business and card issuers and users in developing countries as well. Despite the recent developments in technology
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