with the eluent that is lower in polarity. 2. TLC in today’s experiment stands for Thin Layer Chromatography. 3. From least polar to the most polar: Heptane-Toluene-Acetone-Methanol-Acetic acid ‚ ‚ ‚ ‚ 4. The Rf (Retardation factor) is the ratio of the distance that the compound/mixture traveled to the distance the solvent/mobile phase traveled. 5. From least Rf to the highest Rf: Benzoic acid – Benzaladehyde - n-Decane If the TLC were run with a more polar solvent‚ all the
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not explored in this laboratory. This experiment explores these techniques to identify an unknown amino acid by TLC and analyze food colors using paper chromatography. By using 2.0 mL of dimethoxyethane and 18 mL of absolute ethanol as the developing solution‚ and then spotting each sample of amino acids and an unknown on the silica gel chromatography plate‚ we began the process of TLC plating. The ninhydrin solution was sprayed on the chromatography plate and allowed to dry. After allowing the solution
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Investigation and Preparation of Esterification Lab Introduction Esters are classified as organic compounds commonly derived from carboxylic acids. They are compounded from the reaction of a carboxylic acid with an alcohol in the presence of a strong acid to be used as a catalyst. The formula that represents an ester is R-COO-R where both Rs’ are alkyl groups‚ one which is bonded to an oxygen‚ and the other to a carbon which is double bonded to 1 oxygen and single bonded to the other. Esters
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Esterification and Hydrolysis: Methyl Benzoate by Fisher Esterification Nitration of Methyl Benzoate Jingling Li 2/16/2014 Purpose of the experiment: To understand the mechanisms for Fisher esterification reactions as an equilibrium process and hydrolysis is the reversal reaction of esterification. Nitrate methyl benzoate by an electrophilic aromatic substitution reaction. Summary of procedures: Add sulfuric acid to the mixture of benzoic and methanol‚ heat up the mixture to 65 oC
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IDENTIFICATION OF UNKNOWN SOLUTIONS USING TLC CHROMATOGRAPHY Purpose: To become familiar with the principles and terminology of TLC chromatography to identify an unknown molecule based on comparisons with known laboratory standards and Rf factors. Background Thin Layer Chromatography: Chromatography is a sophisticated method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that
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FUNCTIONAL FOODS & NUTRACEUTICALS LABORATORY REPORT LAB 2: FAT & OIL FROM OIL-SEEDS: PART II THIN LAYER CHROMATOGRAPHY ANALYSIS (TLC) COORDINATORS: ASSOC. PROF. DR. JAMALUDDIN BIN MOHD DAUD ABDUL AZIM ADNAN 1016741 INTRODUCTION TLC chromatography or thin layer chromatography is a type of planar chromatography. TLC is routinely used by researcher in the field of phyto-chemicals‚ biochemistry etc. to identify the components in a compound mixture like alkaloids
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chromatography (TLC) is an extremely valuable analytical technique. It provides a rapid separation of compounds‚ and gives an indication of the number and nature of the components of a mixture. TLC can also be used to identify compounds by comparison with known samples‚ to check the purity of a compound‚ or to monitor the progress of a reaction‚ an extraction‚ or a purification procedure. This experiment will introduce you to the mechanics of TLC‚ and the chemical principles behind it. TLC is normally
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Top 40 hit "Girl Talk"‚ and earned two Grammy Awards nominations. It featured previously unreleased vocals from Left Eye.Billboard magazine ranked TLC as one of the greatest musical trios.[5] Between 1992 and 2002‚ the band had ten top ten singles‚ four number one singles‚ four multi-platinum albums‚ and won fiveGrammy Awards. At the end of 1999‚ TLC was ranked as the seventh most successful act of the 1990s by Billboard. In 2008‚ the group was inducted into the All Time Hot 100 Artist Hall of Fame
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In this TLC lab‚ we explored the relative polarities of Acetylsalicylic Acid‚ Ibuprofen‚ Acetaminophen‚ and Caffeine based on their functional groups. The most overall polar analgesic is considered to be the analgesic that contained the most polar function groups. The amine and amide functional groups are the most polar. In this experiment‚ we found that both these functional groups are found in Caffeine. As a result‚ Caffeine was the most polar out of Acetylsalicylic Acid‚ Ibuprofen‚ Acetaminophen
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synthesize methyl benzoate form benzoic acid and methanol by using the Fischer esterification method. The Fischer exterification technique is utilized in the academic and industrial settings due to the simplified synthesis and safety parameters of the overall reaction. Both benzoic acid and methanol are relatively cheap to obtain from a commercial source as well as being easy to store with a relatively long shelf life. The Fischer esterification method is a fundamental and important synthesis process in training
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