SSN COLLEGE OF ENGINEERING DEPARTMENT OF CHEMICAL ENGINEERING V Semester – CH 2307 TECHNICAL ANALYSIS LAB -2010 INDEX SHEET CYCLE ONE Date of Condn. Date of Subn. Sign 1 Estimation of COD of the given sample of water. 2 Estimation of Manganese in the given pyrolusite ore. 3 Estimation of Magnesium by EDTA method. 4 Estimation of purity of drug using pH meter. 5 Estimation of Ammonia in ammonium salts. 6 Polarimetric estimation of sugar
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Exercise 1 – Potato Change in Mass versus Sucrose Concentrations Introduction: This exercise involves estimating the osmotic concentration of potato tuber cells by using a change in mass method. The null hypothesis states that there will be no change of mass of the potato disks after they have been incubated in any sucrose solution. This means that the concentration of sucrose that the potatoes are in will no effect the movement of water in or out of the potato cells. However‚ the alternative hypothesis
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PRODUCTION OF CITRIC ACID Introduction: Citric acid is a White solid‚C3H4OH(COOH)3‚soluble in water and slightly soluble in organic solvents‚ which melts at 153 oC. Aqueous solutions of citric acid are slightly more acidic than solutions of acetic acid. Traces of citric acid are found in numerous plants and animals‚ because it is a nearly universal intermediate product of metabolism. Large amount of the acid are found in the juice of citrus fruits‚ from which it is precipitated by the
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THE AFFECTS OF DIFFERENT SUCROSE SOLUTIONS ON OSMOSIS OF EGG MEMBRANES A laboratory report submitted for LE@D Biology 1004 Michele Liveley Arkansas State University Mountain Home Mountain Home‚ AR September 2013 Osmosis Egg Lab Report Introduction Osmosis is the passing of water or other solutions from an area of high concentration of particles to an area of less concentration. The cell membrane is an organelle‚ selectively permeable‚ and only
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individual work and is my sole intellectual property. It does not contain the ideas or writing of other individuals/authors. _Alexandra DeWitt_____________ __5/1/13____________ Author Date _10_________ Lab Section # Increasing Sucrose Concentrations Causes the Osmotic Concentrations of Disks of Potato Tubers to Decrease Abstract: Introduction: The biological membranes are composed of phospholipid bilayers‚ each phospholipid with hydrophilic heads and hydrophobic tails‚ and proteins
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A. Osazone Formation Crystals Formed? (hot) Crystals Formed? (Cold) 5% sucrose Yes (orange) Yes (yellow) 5% glucose Yes (orange) Yes (yellow) 5% fructose Yes (brown) Yes (brown) 5% maltose Yes (orange) Yes (gold) B. Molisch Test Color of Junction Glucose Purple Sucrose Purple Starch Purple C. Bial’s Test Color Change Ribose Yellow -> Dirty Green Glucose Yellow -> Dark Yellow D. Seliwanoff’s Test Observation Sucrose 1st (Dark) Glucose 2nd (Medium) Fructose 3rd (Light) E. Benedict’s Test
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that bag into a beaker containing a 1.0 M solution of sucrose‚ the percent of mass lost in the bag is 10.5%. The solution in the bag is hypertonic while the solution in the beaker is hypertonic‚ which is why water moves from the bag to the beaker and the bag loses mass. b. The purpose of this experiment is to see whether or not temperature plays a role in the percent change during diffusion or osmosis. c. If the temperature of the sucrose solution in the beaker increases‚ then the percent change
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dialysis tubing Sucrose solution concentrations of 0M‚ .2M‚ .4M‚ .6M‚ .8M‚ 1.0M String Balance 6 beakers or plastic cups
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I am going to investigate osmosis when potato is placed in different concentrations of sucrose. I am aiming to witness osmosis in 5 different concentrations of sucrose. I will use 5 varying concentrations so that I have a wider spread to compare the results‚ and check that I don’t have any anomalies Prediction Osmosis is the process of diffusion of water molecules from a weaker solution into a stronger solution‚ through a semi permeable membrane. The tiny pores in the membrane
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isotonic with) as the intracellular environment of the cells. Aim The aim of this practical is to determine the isotonic concentration of sucrose in potato cells. Hypothesis If the concentration of the sucrose solution in which the potato cylinder is bathed in changes‚ then there will also be a change in the mass of the potato cylinder. Variables Independent: Sucrose concentration Dependant: Percentage change in mass Other variables in the experiment are the potato which is to be controlled by having
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