SINGAPORE POLYTECHNIC SCHOOL OF CHEMICAL & LIFE SCIENCES CP 4001: ANALYTICAL & PHYSICAL CHEMISTRY Experiment 4: Gravimetric Analysis Prepared for: Mr Goh Tong Hng Submitted by: Ng Hui Shan (0900931) DBS/FT/1A/02 26th May 2009 CONTENTS 1. Synopsis 3 2. Objectives 4 3. Theory 1. Experimental Procedure 4 2. Stoichiometric Calculation 7 4. Procedure 7 5. Results & calculations 1. Amount of
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Unit 2 – Disposition of Toxicants Ch 5 – Absorption‚ Distribution‚ and Excretion of Toxicants Ch 7 - Toxicokinetics Chapter 5 Absorptions‚ Distribution‚ and Excretion of Toxicants © The McGraw-Hill Companies‚ Inc‚ 2011 Factors Affecting Disposition • If fraction absorbed or rate absorbed is low‚ may never reach high enough concentration to cause toxicity • Concentrated in a tissue other than the target organ‚ thus decreasing toxicity • Biotransformation may result in formation of less toxic
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modifications of filtration and dialysis use a matrix-based fibrous material that provides a mechanism of separation in many homogeneous immunoassays. These materials may be coated with specific antibody-ligand to foster selection of specific materials or species. Certain labels use magnetic particles in conjunction with strong magnets to effect separation. Basic universally used separation mechanisms‚ outside of those incorporated in immunoassay‚ are centrifugation‚ filtration‚ and dialysis
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2- Attapulgite clay‚ zeolites and polymer filtration; 3- Nanocatalysts; 4- Magnetic nanoparticles; 5- Nanosensors for the detection of contaminants. Nanofiltration membranes Researchers are using nanomaterials ( e.g carbon nanotubes‚ alumina fibres) to build structures that have controlled shapes‚ density and dimensions for specific filtration applications. For instance‚ researchers have developed and tested cylindrical membranes with pores tiny enough to filter out the smallest organisms
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___ ___ 4. The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO membrane in place. Explain the results. How well did the results compare with your prediction? ___ ___ Activity 4 Simulating Filtration 1. Explain in your own
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g/mole. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer : c. glucose only Stop & Think Questions: The reason sodium chloride didn ’t diffuse left to right is that You correctly answered: c. the membrane pore size was too small. Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mole. There is no reason to run these solutes at the 20 MWCO because You correctly answered:
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Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 or 23 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through?50‚100‚ 200 4. Which materials diffused from the left beaker to the right beaker? Urea‚ NaCl and glucose diffused 5. Which did not? Why? Albumin was too large to diffuse into the right beaker. Activity 2: Simulating Dialysis 6. What happens to the urea concentration
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Lab BCH 2333 Section: Lab 1 Carbohydrates: Separation Techniques Based on Molecular Size TA: Wednesday‚ January 16th‚ 2013 Team #4 By: Partner: Purpose The purpose of this experiment is to exemplify how differences in molecular weight allow separation of polymers from their monomers. Methods of dialysis and gel filtration chromatography will be used to separate a glucose monomer from a starch polymer. Colorimetric
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Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through? 50‚ 100‚ and 200 4. Which materials diffused from the left beaker to the right beaker? NaCl‚ Urea‚ and Glucose at MWCO 200 5. Which did not? Why? Albumin‚ too large to diffuse Activity 2: Simulating Dialysis 1. What happens to the urea concentration in the left beaker
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Cell Transport Mechanisms and Permeability 1 EXERCISE 1 OBJECTIVES 1. To define the following terms: differential permeability‚ passive and active processes of transport‚ diffusion (simple diffusion‚ facilitated dif- fusion‚ and osmosis)‚ solute pump‚ pinocytosis‚ and phagocytosis. 2. To describe the processes that account for the movement of sub- stances across the plasma membrane‚ and to indicate the driving force for each. 3. To determine which way substances will move passively
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