SOP: Solid phase extraction of cylindrospermopsin in filtered water samples Document identifier: SOP_TOXIC_UDU_05F Prepared by: James S. Metcalf and Geoffrey A. Codd‚ UDU Date: 7 July 2005 1 Introduction In order to determine the concentration of cylindrospermopsin in the extracellular fraction of filtered environmental waters‚ solid phase extraction (SPE) is necessary to concentrate the toxin to concentrations capable of being detected by HPLC. 2 Experimental 2.1 Materials
Premium High performance liquid chromatography Chromatography Glass
called extraction. Extraction is a systematic process of separating mixtures of compounds‚ taking advantage of the affinity differences of compounds to separate them (Padias 128-37). This technique recognizes the principle that “like dissolves in like‚” that is‚ polar solutes dissolve in polar solvents and non-polar solutes dissolve in non-polar solvents. Through extension of this principle‚ one can use extraction to separate compounds of a mixture. There are three different methods of extraction; solid-liquid
Premium Solvent Solubility
Berry DNA Extraction Lab I. Problem Statement: Successfully extracting the DNA from strawberries. II. Background info: DNA is the genetic information in organisms that codes for proteins‚ and controls the cells function. The DNA is a double helix molecule that is formed by various nucleotides which include Adenine‚ Thymine‚ Guanine‚ and Cytosine with sugar phosphate. Different organisms can have larger or smaller genes. DNA is also what makes up chromosomes during cell replication. Strawberries
Premium DNA
Introduction Extraction is a purification technique used in organic chemistry to separate compounds from a mixture of two or more compounds. There are three different extraction techniques: liquid-liquid extraction‚ solid-liquid extraction and chemically active extraction. All three types of extraction follow the same principle. Organic molecules dissolve in organic solvents and polar molecules dissolve in aqueous solvents. This phenomenon is observed because of the intermolecular forces between
Premium Solubility Solvent Chemistry
its mixture using acid-alkaline extraction. 2. To determine the percentage recovery and melting point of benzoic acid and p-diclorobenzene. Apparatus:Separatory funnel(250mL)‚Buchner funnel‚beaker. Materials:Benzoic acid‚p-dichlorobenzene‚ether‚10% NaOH‚conc.HCl‚distilled water‚ anhydrous CaCl2. Introduction: Organic compounds in an aqueous mixture can be separated by shaking the aqueous solution with a solvent which is immiscible with water. Extraction involves dissolving either a
Premium Sodium chloride Sodium hydroxide Hydrochloric acid
’ is the embryo‚ which is the part of the seed that can grow into a new wheat plant. (1) Aim: To extract DNA from Wheat Germ cells‚ and learning the DNA extraction process. Hypothesis: Each step of the process will help in a certain way to extract DNA‚ until we are finally successful in the end. In the wheat germ extraction‚ the ethanol will be able to separate the DNA‚ and it will float between the less dense ethanol and the denser homogenizing mixture. (2) Materials: 1 gram
Premium DNA Gene
The purpose of the DNA extraction lab was not only to inform students on how DNA is present in humans and all organisms‚ but to also educate them on how DNA can be extracted using common household materials. Also‚ the lab was very efficient as it introduced the students to extracting their own DNA found on their cheek cells as well as letting them take an observation on how DNA appears or how it is formed. Additionally‚ students were instructed through a very clear and simple procedure‚ which guided
Premium Education Learning DNA
THE EXTRACTION OF DNA THE EXTRACTION OF DNA 07/02/2013 | By Laura Green‚ Tasnim Rahman and Sandra Strammiello | Table of Contents DNA………………………………………………………………………………………..………… 2 The Discovery of DNA…………………………………………………………………………...…………..…………. 4 The Steps for Extracting DNA…………………………………………………………………………………………….…..… 5 Experiment: Which Product Can the Most Visible Amount of DNA Be Extracted From?..............................................................................................
Free DNA
Abundance and Extraction of Metals 1. a Mineral - A mineral is a naturally occurring inorganic solid‚ with a definite chemical composition‚ and an ordered atomic arrangement. b Ore - Deposit in the Earth of valuable metals contained in minerals. c Economic Natural Resource Deposit - This is a natural deposit of something that is economically viable to mine‚ refine and sell. Hence the deposit must be big enough to last for a large period of time‚ so that it will make enough money for the
Premium Aluminium Oxygen Mineral
Definition of Terms The following terms were defined for the readers to have a better understanding of the reserch paper. Active constituent. It refers to the component of the plant responsible for the exhibitory activity of the drug.1 Agar. It is gelatinous colloidal extractive of a red alga (as of the genera Gelidium‚ Gracilaria‚ and Eucheuma) used especially in culture media or as a gelling and stabilizing agent in foods.2 Agar-well diffusion method.3 It is a method of bioassay wherein
Premium Yeast Bacteria Fungus