The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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Watson and Crick discovering DNA Watson and Cricks discovery of DNA was important for many reasons because DNA made it possible to use genetic screening to identify which patients have ‘faulty’ genes which will lead to genetic illness such as Alzheimer’s. Watson and Crick discovered the structure of DNA in 1953. A reason that it was important was because it has provided an explanation for diseases and conditions that the germ theory cannot explain‚ for instance‚ cancer. This was important because
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The Role of DNA in Protein Synthesis DNA is found in the cells nucleus. It is part of the chromosome of the cell. DNA is the molecule that controls the making of protein. Protein is used for the growth of cells and organisms and also for the repair of cells. Protein is also used as an enzyme to speed up reactions in the body. Because of this DNA is able to control the whole organism. With out DNA and its ability to make protein the cells and eventually the whole organism would die
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Title: DNA analysis Aim: a) Isolate and Purify Bacterial Chromosomal DNA from a strain of E.coli b) Visualization of restriction fragments by Agarose Gel electrophoresis Objectives: * to isolate and purify bacterial chromosomal DNA from a strain of E.coli * to analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different
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Extraction of Caffeine from Tea Leaves Department of Biological Sciences‚ College of Science University of Santo Tomas España‚ Manila‚ 1028 ABSTRACT: Caffeine is a white crystalline xanthine alkaloid that acts as a stimulant drug. Caffeine is found in varying quantities in the seeds‚ leaves‚ and fruit of some plants. It is most commonly consumed by humans in infusions extracted from the seed of the coffee plant and the leaves of the tea bush‚ as well as from various foods and drinks containing
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radically expanded. Technological developments such as DNA profiling and how investigations are conducted has significantly improved humankinds ability to investigate and solve crimes through everyday science. The use of DNA evidence in criminal investigations has helped law enforcement identify criminals and solve difficult crimes. DNA can also be used to clear the accused and free people who are wrongly accused or convicted of crimes. DNA was first described by scientists Francis Crick and James
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are very helpful to you as you can see. Last but not least are nucleic acids. They consist of DNA‚ RNA‚ ATP etcetera. A difference between DNA and RNA is that DNA is double stranded and RNA is single stranded. The monomer of it is nucleotides. The structure is made up of sugar‚ phosphate group and a nitrogen Functions include the DNA containing all of your hereditary information and RNA uses your DNA to make proteins. This
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Pure aluminium is a relatively soft‚ silvery white metal. When exposed to air‚ a thin coating of Aluminium oxide gives it a dull lustre. It is three times less dense than water‚ and has great strength when alloyed‚ it doesnt rust and has high electrical conductivity‚ and Aluminium is also ductile‚ making it a very useful metal. Aluminium readily makes alloys with copper‚ zinc‚ magnesium‚ manganese and silicon. Aluminium foil is 92-99% pure aluminium. Other uses of aluminium alloys include computers
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°Thioridazine hydrochloride‚USP. Is a member of the piperizine subgroup of the phenothiazines. Thioridazine occurs as a white or slightly yellow‚ crystalline or micronized powder‚ which is odourless or has a faint odor and is practically insoluble in water and freely soluble in dehydrated alcohol. The drug has sedative and hypotensive activity in common with chlorpromazine and less antiemetic activity. thioridazine is indicated for the treatment of schizophrenic patients who fail to respond
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OBJECTIVES The Basis of Heredity 1. Explain in general terms how traits are transmitted from parents to offspring. An organism’s traits and appearance are controlled by specific forms of proteins contained in DNA. When gametes are formed‚ the male and female parents pass along their DNA into their respective gametes. Fertilization fuses the gametes and combines the genetic contribution of each parent into the new offspring. 2. Distinguish between asexual and sexual reproduction. Asexual Reproduction-A
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