Extraction of DNA Science Fair Project 2013-2014 Produced by Nadia Walker Due Date: Monday January 20th 2014 Presented to Mr. Freimann Date Submitted: Monday January 20th 2014 Table of Contents Introduction page 1 Hypothesis and materials
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My hypothesis is that if I turn the water to vapor then I will be able to determine the ratio and the amount of moles of the salt and water. This is because by using evaporation I will be able to learn the mass of the salt and of the water needed for n=m/MM which states the amount of moles can be found by dividing mass of the moles by the molar mass. Experiment 1 First I took a crucible‚ a balance‚ and a Bunsen burner and placed them onto the workbench. Then I measured the mass of the empty crucible
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Extraction (Ch # 07) Faraz Gohar Mr. Wayne Xie Chem 201 10/02/2012 Objectives : 1)To purify samples of organic compounds that are solids at room temperature. 2)To dissociate the impure sample in the minimum amount of an appropriate hot solvent Chemical Index : Naphthalene; mp 82 *C 1‚4-Dichlorobenzene; mp 56 *C 4-Chloroaniline; mp 68 - 71 *C; pKb 4.15 Ethyl 4-Aminobenzoate; mp 90 *C; pKb 4.92 Procedure :
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Objective : To extract the DNA from onion samples. Introduction : Nuclues has DNA molecule in it. It is packaged into thread like structure known as chromosomes. Each and every chromosome is made up of DNA tightly coiled many times around proteins which is known as histones. Histones supports the structure. Chromosome cannot be visible even under the microscope if the cells are not dividing. DNA consist of 2 long polynucleotide which is composed of 4 types of nucleotide units. Nucleotides
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Technique of Water Evaporation Introduction When water evaporates from water storages it becomes a major issue that affects regions of agriculture in most lands leading to annual loss exceeding potentially to 40 precent and more of water storage. This water which is lost leads to reduction of productivity in agriculture affecting the environment adversely. Literature review has been developed on the same by various researchers in order to develop evaporation suppression techniques
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METHODS OF EXTRACTION FOOD PROCESSING PRACTICAL (TFT 709) BY GROUP THREE MEMBERS ISMAILA AYUBA RAMADAN Submitted to Department of Food Technology University of Ibadan Lecturer-in-charge Dr. Akinoso Febuary‚ 2012 Abstract Oil extraction was carried out using two different extraction method (water and solvent) and the yield for different method were obtained. The free fatty acid for different oil was also determined immediately after extraction and also
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techniques of an acid base extraction‚ recrystallization‚ and use of Melt Temp (melting point range). The reaction of an unknown acid (3-chlorobenzoic acid) with diethyl and sodium hydroxide was observed‚ and the solution naturally separated into an aqueous and organic solution. The mixture of acid and organic solution was created and then separated through the Hirsch funnel into separate beakers of an aqueous and organic solution. The neutral compound was obtained through evaporation‚ while the unknown
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Of a Sample Mixture By Liquid-Liquid Extraction Reading assignment: Techniques in Organic Chemistry 2nd ed pages 75-99. 3rd ed pages 113-140. Topics and Techniques i) identification of solvent layers of two immiscible solvents ii) partioning of a compound between two immiscible solvents and determination of KD iii) liquid-liquid extraction with aqueous acids and bases with organic solvents. iv) use of drying agents Introduction Liquid-liquid extraction is a method used for the separation of
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called extraction. Extraction is a systematic process of separating mixtures of compounds‚ taking advantage of the affinity differences of compounds to separate them (Padias 128-37). This technique recognizes the principle that “like dissolves in like‚” that is‚ polar solutes dissolve in polar solvents and non-polar solutes dissolve in non-polar solvents. Through extension of this principle‚ one can use extraction to separate compounds of a mixture. There are three different methods of extraction; solid-liquid
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