Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of substrate (sucrose)
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Enzymes Enzymes are the sparks that start the essential chemical reactions our bodies need to live. They are necessary for digesting food‚ for stimulating the brain‚ for providing cellular energy‚ and for repairing all tissues‚ organs‚ and cells. There are three types of enzymes: metabolic enzymes‚ digestive enzymes‚ and food enzymes. Metabolic enzymes catalyse‚ or spark‚ the reactions within the cells. The body’s organs‚ tissues and cells are run by metabolic enzymes. Without them our bodies
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Biology “Enzyme Activities” Introduction: Enzymes have extremely interesting properties that make them little chemical-reaction machines. The purpose of an enzyme in a cell is to allow the cell to carry out chemical reactions very quickly. These reactions allow the cell to build things or take things apart as needed. This is how a cell grows and reproduces. At the most basic level‚ a cell is really a little bag full of chemical reactions that are made possible by enzymes (Brain). Laboratory
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There are many reasons why enzymes have such a high specificity. The first variable is an enzyme’s primary structure. A primary structure is just a combination of amino acids. There are twenty different amino acids that the primary structure can be created from. Every enzyme has a different order that the acids are placed in and each one has a different number or amino acids. The slightest change in this structure can affect a protein’s conformation and function. The secondary structure is a regular
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Enzymes play a vital role in helping our body function. They act as biological catalysts and help speed up reactions that would otherwise take long periods of time to naturally occur. Enzymes help lower the activation energy required for the reactants to reach the transitional state from which then they can form products. However‚ enzymes do not change the free energy of the reaction. Enzyme’s ability to catalyze reactions comes from the shape of the active site on the enzyme. Enzymes are hyper-specific
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Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all very specific as each enzyme just performs one particular reaction. Catalase
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Lab report nº4 The aim of this experiment was to observe the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%‚ 80%‚ 60%‚ 40%‚ and 20% According to P.George: “When catalase is added to hydrogen peroxide‚ there is an initial rapid evolution of oxygen which lasts for about two minutes‚ depending on the peroxide concentration. After this‚ oxygen is given off at a steady rate which
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Running head: LAB 5: INVESTIGATING AN ENZYME-CATALYZED REACTION Lab 5: Investigating an Enzyme-Catalyzed Reaction September 24‚ 2014 Principles of Biology 120.601 Mrs. Annemarie Duncan Abstract: (Burmania) This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in
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of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature of the reaction on the pectinase
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Procedures for Part A: For Activity A‚ we first tested enzyme activity. First‚ we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then‚ we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that‚ we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that‚ we placed the test tube filled
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