CORE PRACTICAL ONE Describe how the effect of caffeine on heart rate in Daphnia can be investigated practically‚ and discuss whether there are ethical issues in the use of invertebrates. Daphnia‚ the water flea‚ is a small freshwater crustacean which lacks physiological methods of maintaining a constant body temperature. This means that if the environmental temperature changes‚ its body temperature does so too and its metabolic rate will be expected to rise or fall accordingly. So the temperature
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practical was to observe what effect temperature had on the permeability of the cell membrane. Introduction Located within the vacuole of beetroot cells is a red pigment call Betalains. Typically these pigments are contained within the cell vacuole by the tonoplast (vacuole membrane)‚ however When beetroot is heated these red pigments escape the vacuole. This experiment aims to explore the effect of temperature on the permeability of the cell membranes (i.e. Tonoplast). ‘The cell surface
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Beetroot Cells with High temperatures and low pH solutions Friday 15th March 2013 Abstract (50) The aim of this investigation was to study the effects of temperature and a low pH solution on beetroot cells. Introduction (200) The general belief amongst our group members was: an increase in temperature will damage the permeability of the plasma membrane. Also‚ the heat will break away the permeability. If the pH solution is 7 or greater there will be no change but the lower the pH the more proteins
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Lab Report: To investigate the effect of temperature of the permeability of cell membranes DCP (Data Collection and Processing Section) Raw Data Absorbance of the colored beetroot solution at 565 nm of different temperatures of 30℃‚ 40℃‚ 50℃‚ 60℃‚ and 70℃ Temperature (℃) (± 0.1℃) Absorbance of the colored beetroot solution at 565 nm (± 0.001 AU) Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 30 0.160 0.129 0.136 0.135 0.128 40 0.481 0.343 0.376 0.491 0.410 50 0.386 0.597 0.378 0.743 0
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Abstract: Introduction: The permeability of a cell to solutes in an aqueous medium depends upon the physical and chemical make–up of the membrane. The maintenance of the living cell depends upon the continued presence and functioning of a selectively permeable membrane. If the nature of the membrane is changed or altered in any way‚ this may well affect its permeability and thus the properties of the cell of which it is a part. Irreversible changes in the permeability of the membrane usually lead
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Investigating the effect of temperature on the permeability of the plasma membrane of beetroot tissue. Aim- To determine the temperature at which the beetroot plasma membrane is destroyed by heat. Table of results: Temperature of water bath(degrees Celsius) Absorbance(au) (+ or – 0.06au) Repeat 1 Repeat 2 Repeat 3 Mean of results. 30 0.5869 0.6029 0.5946 0.5948 40 0.4161 0.4436 0.7056 0.4298 50 0.4343 0.4077 0.4137 0.4185 60 0.3486 0.4466 0.7991 0.3976 70 1.0500 0
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Apparatus and materials: 1 tube mouth rinse(4 brands)‚ 3 cylinders beetroot‚ 1 pc razor blade‚ 1 pc white tile‚ 10 pcs test tubes‚ 2 pcs test tube racks ‚ 1pc 10-mL measuring cylinder‚ 5 pcs labels ‚ 1 pc forceps‚ 1 pc 500-mL beaker Aim : To study the effect of different brands of mouth rinse on the permeability of the membranes of beetroot tissue Problem: To compare the denaturation of membrane of cells in the effect of mouth rinse of different brands and find out which brand of mouth rinse
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membrane. Furthermore with heat lipids become more fluid making the membrane fragile. In beetroot when the membrane is damaged‚ the red pigment it contains is released. Variables
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Hypothesis: Increasing the temperature and pH levels which the beetroot membrane cells are exposed to will increase the amount of dye that passes out of the membrane. Materials: Part A • Bunsen Burner‚ Tripod and a Wire Gauze • 250mL Beaker • A 100° thermometer • Matches • 10mL measuring cylinder • Sticky labels and a Pen • Fine forceps • Eight test tubes • Test-tube rack • Access to fridge and freezer • 30 washed beetroot slices in a 100mL beaker of water • Knife Part B • 10 Test tubes
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EFFECT OF CHANGES IN TEMPERATURE AND SDS CONCENTRATION ON THE STRUCTURE OF BEETROOT CELL MEMBRANES. Introduction Cellular Membranes or cell membranes are biological membranes that separate the interior of a cell from the outside environment. Cell membranes are selectively permeable to ions and some molecules and control the movement of substances in and out of the cell. (Danyk‚ 2013/14) Cell membranes separate and organize chemicals and reactions within cells by allowing selective passage
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