Unit 4 Lab – Effects of Osmosis on Living Tissue Assignment Total: 35 Points Course Weight: 6 Pre-Lab Preparation - Review the following resources to complete this assignment: Textbook: Chapter 5‚ pg. 83-86 Mastering Biology / Study Area / Chapter 05 – The Working Cell / Student Home Key Concepts Video: Plasmolysis Video: Turgid Elodea Activity: Membrane Structure Activity: Diffusion Activity: Osmosis and Water Balance in Cells Bioflix: Membrane Transport Additional Resources Blast Animation: Diffusion
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Experiment: Turgor in Potato Tissue 1. We labelled three test tubes A‚ B‚ C‚ D and added initials of people in our group. 2. Used a syringe or graduated pipette to put 20 cm3water in tube A and 10 cm3 in tube B. 3. Put 20 cm3 of 17% solution in tube C and 10 cm3 in tube B; put 20 cm3 pure sucrose solutions in tube D. 4. Working on the dissecting board I cut the ends off large potato. I was careful with the knife and cut it onto the dissecting board away from myself. 5. Used a cork
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Osmosis in Potato Tissue Experiment Background Information Osmosis can be defined as the movement of water across a semi-permeable membrane from a region of high water concentration to a region of low water concentration. The semi-permeable membrane allows small particles through it but does not allow large particles such as sodium chloride. Osmosis will continue until a state of equilibrium is reached i.e. there is no area with a higher or lower concentration than another area
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known as osmolarity‚[1] is the measure of solute concentration‚ defined as the number of osmoles (Osm) of solute per litre (L) of solution (osmol/L or Osm/L). The osmolarity of a solution is usually expressed as Osm/L (pronounced "osmolar")‚ in the same way that the molarity of a solution is expressed as "M" (pronounced "molar"). Whereas molarity measures the number of moles of solute per unit volume of solution‚ osmolarity measures the number of osmoles of solute particles per unit volume of solution
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GLADSTONE ROAD AGRICULTURAL CENTRE CROP RESEARCH REPORT NO. 1 TUBER QUALITY AND YIELD OF FOUR SWEET POTATO VARIETIES EVALUATED DURING 2007 Kenneth VA Richardson Department of Agriculture Nassau‚ Bahamas November 2009 ABSTRACT A variety trial was conducted on four sweet potato varieties from April to October 2007 at the Gladstone Road Agricultural Centre. The variety ‘Six Weeks’‚ which is an early maturing variety with white flesh and high dry matter content‚ produced the highest marketable yield
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concentrations of 0.0 mol dm3‚ 0.2 mol dm3‚ 0.4 mol dm3‚ 0.6 mol dm3 and 0.8 mol dm3 affect the osmolarity point of potato cells‚ where it is investigated how osmosis changes the weight of the potato cells‚ measured by the percentage change between the initial and final mass of the potato? Independent Variable: Increasing sucrose concentrations Dependant Variable: Mean percentage change in mass of potato cells All cells have a protective barrier‚ one which selectively allows substances to enter
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OF POTATO TUBER CELLS. Method. Five sucrose solutions with varying molarity and one control containing distilled water were prepared and poured into test tubes. The potato discs were dried‚ weighed and added to the test tubes. The discs were then weighed again after a period of 24 hours. The percentage change in mass was then calculated. Apparatus.  Specimen tubes with stoppers x6  1cm3 diameter cork borer  razor blade  filter papers 
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Results The relative osmotic concentration was determined by measuring the percent change in mass of the potato tissues. Change in mass was measured of six solutions‚ each containing different levels of concentration (0‚ 0.1‚ 0.2‚ 0.3‚ 0.4‚ and 0.5). The percent change in mass decreased as sucrose concentration increased‚ therefore‚ relative osmotic concentration also decreased as sucrose concentration increased. However‚ the osmotic concentration of 0.2 M sucrose solution was relatively greater
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Based on the result‚ what is known now is when osmolarity is low‚ the hemolysis is high; while when osmolarity is high‚ the hemolysis is low. This supports the hypothesis if the erythrocytes were placed into a high osmotic solution‚ then the erythrocytes would have a lower hemolysis percentage. As shown in graph 1‚ when the erythrocytes were placed into the tube containing 250 to 390 osmolarity of solution‚ the erythrocytes results in below 26 hemolysis percentage. When the erythrocytes were placed
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Estimating Osmolarity by Change in Weight By: Sondus Kahil 27 February 2012 TA- Ashley Hintz Biology 200A-section 002 Abstract: My group and I conducted the experiment that estimates osmolarity by change in weight of potato tubers‚ this was conducted in order to explore the process of diffusion and osmosis and more importantly to investigate the question of “Does different concentrations of sucrose solutions have an effect on the final weight for the potato
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