Extract and Garlic (Allium sativum) Bulb Extract against Escherichia coli Is submitted to Mrs. Merlyn M. Gamboa In partial fulfillment of the requirements in Chemistry Submitted by: Del Rosario‚ Lea Joy Salih‚ Chandler Jay Serrano‚ Ayra Mari Sumayang‚ Noel Taguinod‚ Joshua Cedric III – Meyer Date: January 7‚ 2013 Chapter 1 Introduction Background of the Study Escherichia coli is a Gram-negative‚ rod-shaped bacterium that is commonly found in the
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Escherichia coli Microbiology Lab NAS 185-14L Introduction In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is
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Citrus sinensis on 5 bacteria strains. | 5. State the hypothesis (es)‚ if applicable | | 6. Statement of objectives | To evaluate the antibacterial activity of the crude leaf extract of Citrus sinensis against bacteria strains like Escherichia coli‚Pseudomonas aeruginosa ‚ Klebsiella pneumoniae‚ and Staphylococcus aureus.To be able to determine in which extract (aqueous or ethanol) the antibacterial capacity of Citrus sinensis is most effective. | 7. Variables used – independent and
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fixed oil was investigated. Agar cup diffusion assay was performed against two fungal species (Candida albicans and Aspergillus niger)‚ two gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Ampicillin‚ gentamycin and clotrimazole were used as positive controls. Antimicrobial activity was shown by all the species except E.coli and P. aeruginosa. Oil showed a highest activity against Candida albicans with
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coli utilize a number of virulence factors to adhere the uroepithelial cells (Vranes J et al.‚ 2001 and Johnson JR 1991). The longer the urinary catheter remains in place‚ the greater is the tendency of these organisms to develop biofilms‚ which may result
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except for the TDA‚ IND and VP SAC Blue coloured suspension formed - MEL Blue coloured suspension formed - AMY Blue coloured suspension formed - ARA Yellow coloured suspension formed + • Result of API 20 E refers to the presence of Escherichia coli 1 and Escherichia 2 in the bacterial suspension. Analytical profile index which is also known as API is generally used for rapid identification of classified microorganisms. This API 20E kit
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phytochemical analysis. Specifically‚ it answers the following: 1. What are the phytochemical content of the stickcane shoot extract? 2. Is there a significant difference in the antibacterial properties of the plant extract on Pseudomonas and Escherichia Coli as shown by the zone of inhibition? 3. Does the stickcane shoots extract exhibit antibacterial property? Significance of the Study The result of this study can be beneficial to the community. It can motivate the mass production of
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Deaminase)‚ Lysine Decarboxylase‚ and Ornithine Decarboxylase. Colonial morphology on EMB was used to prove the identity of the bacteria. The unknown bacteria was #9. After performing the tests it is determined that the unknown bacteria is Escherichia coli. 3. Methods: All tests were performed using the aseptic technique to assure the cultures were pure and not contamination was present. The inoculation loops and straight wire stab needles were placed over the flame of a Bunsen burner and heated
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Activity of Cilantro on Staphylococcus Epidermis and Escherichia coli Abstract Antimicrobial effects of cilantro against the gram positive bacteria S. epidermis and the gram negative bacteria E. coli were looked at. We predicted that cilantro would act as an antibiotic against S. epidermis but not to E. coli because S. epidermis does not contain an outer membrane whereas E coli. does. We predicted that the semi-permeable outer membrane of E. coli would protect the bacteria from any antimicrobial
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Journal of Experimental Microbiology and Immunology (JEMI) Vol. 14: 98-100 Copyright © April 2010‚ M&I UBC The Effects of the Ratio of Surface Area to Volume on Transformation Efficiency in Escherichia coli K-12 Eliana Pouchard‚ Steve Teng‚ and Cameron Wong Department of Microbiology & Immunology‚ UBC In the process of transformation‚ bact eria take up DNA fr om the environment through their cell wall. To induce competence in the cells‚ the DNA for uptake must first attach
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