Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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CHAPTER 4 RESULTS AND DISCUSSION 4.1. Angelika keiskei (Ashitaba )crude non-polar extract The crude non-polar extract from the plant Angelika keiskei (Ashitaba) was tested for the anti-bacterial property by using the Kirby – Bauer Disk Diffusion test. There were four 25uL of Ashitaba non-polar extract with different concentrations used which were 12.5 %‚ 25%‚ 50% and 100%. In line with this‚ respective positive controls of the two specimens and negative control were also used. The results
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combat various physiological and pathological disorders in humans. If the antibacterial protein is isolated and is proved non-cytotoxic to humans‚ then it can be researched and developed further as a drug for curing various diseases caused by Escherichia coli and Staphylococcus aureus ailments in human beings and thus give an impetus to further research on being used as model organisms to be studied. 5.2 DEFINITION OF THE PROBLEM: Treating every bacterial-caused
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Biology June 13‚ 2011 E. coli Levels in the Finley River and the Possible Effects on the Recreational Users. Significance: E. coli ‚ short for Escherichia coli‚ is a class of bacteria found naturally in the intestines of humans and other land dwelling animals (6). It is part of a group of bacteria known as coliform. There are hundreds of known strains of E. coli. Most are harmless. Some however‚ such as E. coli O157:H7‚ can cause severe infection which can often cause
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Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
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Study of Bacterial Growth and Resistance Level to Certain Antibiotics INTRODUCTION Escherichia coli—better known as E. coli—is a gram negative‚ rod shaped bacteria. It is relatively harmless‚ but can occasionally cause food poisoning. It can also provide Vitamin K2. It prevents the establishment of pathogenic bacteria‚ and is associated with or found in the intestinal organ. The antibiotic that E. coli is resistant to is Penicillin. Bacillus subtilis—better known as B. subtilis—is known as
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Kamias (Averrhoa bilimbi) LEAVES AND FRUIT EXTRACT: ITS ANTIBACTERIAL EFFECT ON Escherichia coli‚ Staphylococcus aureus AND Salmonella enteritidis A Science Investigatory Project as an Entry for the 2008 School Level Science Fair Ellaine
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mechanism of action for cranberries is preventing bacteria to bind to “host cell surface membranes”. The bacteria that causes a urinary tract infection is Escherichia coli‚ which is more commonly called E. Coli. Cranberries contain phenol compounds known as proanthocyanidins. These proanthocyanidins‚ in particular‚ “inhibits the Escherichia coli bacteria from attaching to the lining of the urinary tract.” (Lynch‚ 2004) So whether it is a glass of cranberry juice‚ a cranberry cocktail‚ or a cranberry
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Genetic transformation of Escherichia coli with pGLO (Adapted from: Biotechnology Explorer: Bacterial Transformation: The pGLO System. Instructors Guide. BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms.
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Introduction: Escherichia coli is a bacterium that can affect our health or even kill. Like most bacteria‚ E. coli is able to change and progress into different forms based on genetic changes that they can go through. One example of this genetic change is shown in the E. coli becoming immune to ampicillin is blood infections. Because ampicillin has been used so frequently to fight the symptoms of an E. coli infection‚ the bacteria has been able to change itself genetically by producing more of
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