Department of Education Agusan National High School Caraga Administrative Region Division of Butuan City Antimicrobial Activity of Banana (Musa sapientum Linn.) Inflorescence Ethanolic Extract against E. coli and S. aureus Armona Jean B. Ongtico Mel Algil A. Agra Jerrson F. Oronan Researchers’ Mrs. Letecia S. Villanueva Research Coach Abstract The experience of human misery in the form of disease is perhaps as old as the inception
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E. Coli Transformation with Plasmid (pGal)‚ pGal Isolation‚ and Analysis of Plasmid DNA Felicia Osadi Bio 22 April 20‚ 2012 Transformation = group 10 Plasmid = group 7 RFLP = group 1 RESULTS Table I. Plasmid Transformation of E. Coli. Plate # | Agar plate | Type | Result | 1 | X-gal | Control | Extensive lawn growth | 2 | Ampr / X-gal | Control | Clear no bacterial growth | 3 | Ampr / X-gal | Transformation | 1 blue colony | Transformation efficiency = 1 transformants
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Techniques by observing E. Coli and S. Pyogenes under the Compound Light Microscope INTRODUCTION: A German bacteriologist‚ Dr. Theodore von Escherich‚ was the first man in 1885 who discovered the bacterium named Escherichia coli‚ which are gram negative and appears in rod shaped. Most kind of bacteria E. Coli does not cause diseases and some strains indeed are beneficial in helping the process of food breaking down in the intestines. However‚ “the most infamous strain E. Coli O157:H7”‚ which caused
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then over time it should increase more rapidly throughout what is called the log phase. This predictable pattern of growth can be called the organism’s biotic potation. Procedure During this exercise the population growth of the bacteria Escherichia coli (E.coli) will be measured. If the conditions are right‚ the E.coli population will grow more rapidly the longer it stays in that area. Turbidity‚ meaning cloudiness‚ will be how the tubes are measured to give the results of bacterial growth.
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Case Study Luke Tudor 09 Dec 2009 Microbiology‚ Vectors and Control DMS1515 Concerns have been raised about the safety of drinking water being provided for a large temporary community. The area is remote‚ rural‚ without proper sanitation and there are limited multipurpose water resources How could you ascertain the safety of the water? Potable water is fit for consumption by humans and other animals. Water may be naturally potable‚ as is the case with pristine
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experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the transformants were positive for the gene expression. The transformed E. coli on the media appeared fluorescent green under UV light. Introduction The bacteria used in this experiment is Escherichia coli which is not naturally competent. E. coli is a gram negative
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does not belong to the Enterobacteriaceae group? a. Shigella flexneri b. Eschericia coli c. Proteus mirabilis d. Pseudomonas aeruginosa 3. Name the four species of Shigella. 1. Shigella sonnei 2. Shigella dysenteriae 3. Shigella flexneri 4. Shigella boydii 4. Colonies that appear red with black centres on XLD are likely to be which of the following organisms:- a. Shigella flexneri b. Escherichia coli c. Salmonella typhimurium
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single line of each culture Bacillus subtilis‚ Pseudomonal aeruginosa‚ and Escherichia coli‚ to each of the separate sections. The plate was incubated until the next lab period‚ where we then flooded the plate with Iodine and were able to observe and record starch Hydrolysis. (Table 1) * In order to test carbohydrate catabolism under anaerobic conditions we tested cultures of Pseudomonas aerugenosa‚ Escherichia coli‚ and Alcaligenes faecalis in tubes containing a glucose medium. After inoculating
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Imperata cylindrica as an Antibacterial Agent for Escherichia coli INTRODUCTION a. Background of the Study We all know that bacteria are everywhere. Bacteria are microscopic organisms whose single cells have neither a membrane-enclosed nucleus nor other membrane-enclosed organelles like mitochondria and chloroplasts. One example of bacteria is E .coli is a bacterium that is commonly found in the gut endotherms. E. coli and related bacteria constitute about 0.1% of gut flora‚ and fecal-oral
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given mixture of Bacillus cereus‚ Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded‚ clusters‚ singly Small - Staphylococcus aureus Bacteria 2 Gram negative (pink) Rods (shorter than B. cereus) Rounded‚ regular‚ pair‚ clusters Medium - Escherichia coli Bacteria 3 Gram positive (purple) Long
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