absorbansi 0‚08 – 0‚13 untuk mendapatkan standar kerapatan bakteri pada 1 – 2 x 108 CFU/ml (EUCAST‚ 2009; Fransco et al.‚ 2006). 4.8.4 Pengujian Efek Antimikroba Pelaksanaan Uji efek natimikroba menggunakan metode difusi cakram Kirby Bauer. Suspensi Escherichia coli yang telah diukur kepadatannya di swab dengan menggunakan kapas lidi steril secraa mrata pada media MHA (Mueller Hinton Agar). Selanjutnya kertas cakram yang sceraa terpisah telah direndam ekstrak saun biji jambu merah dengan konsentrasi diletakkan
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gram negative and gram positive microorganisms. Materials: Microscope‚ Bunsen burner‚ glass slide pipette‚ loop‚ distilled water‚ nutrient agar of Escherichia coli‚ nutrient agar of Staphylococcus aureus‚ crystal violet‚ gram iodine‚ ethanol‚ safranine‚ oil immersion‚ a piece of napkin ‚ holder. Method: As per manual. Bacteria stained- Escherichia coli and Staphylococcus aureus. Results: Gram-negative bacteria are bacteria
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surrounding the colonies after addition of iodine on PDA plate. Organism Result + or - Appearance/Observations Bacillus cereus Escherichia coli B. Gelatin Hydrolysis (gelatinase production) Note: whether the gelatin deep remains solid or has been liquefied Organism Result + or - Appearance/Observations Bacillus subtilis Escherichia coli C. Coagualse: identification of Staphylococcus aureus Note: whether the rabbit plasma has remained liquid or become solid
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Aims The purpose of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli‚ Pseudomonas fluorescens‚ Enterococcus faecalis‚ Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5‚ 25‚ 37‚ 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different
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jejuni colonies and microscopy * Campylobacter jejuni SEM * Capnocytophaga sp. * Chryseobacterium indologenes on Mueller-Hinton * Chryseobacterium indologenes colonies * Citrobacter freundii colonies on MacConkey * Escherichia coli on blood agar | * Escherichia coli on MacConkey *
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Hydrolysis Report Bile Esculin Hydrolysis‚ Starch Hydrolysis‚ UreaHydrolysis‚ Casein Hydrolysis Hydrolysis Report Introduction Hydrolysis is a chemical reaction that uses water to split complex molecules. The water molecule H2O is split in the mechanism of hydrolysis‚ hydrogen cations and hydroxide anions. When the enzyme catalyzes its reaction inside the cell‚ it is referred to as intracellular hydrolases. When the enzymes secreted from the organism to catalyze reactions outside the cell‚ it
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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coli). Bacillus subtilis is a gram-positive rod-shaped bacterium‚ typically found in vegetation and soil. (Entrez Genome Project). E. coli is gram-negative bacterium commonly found in the gut of warm-blooded organisms. (National Health Service) HYPOTHESIS If we were to compare the antibacterial effects of various antibiotics
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Escherichia coli O157:H7(E-Coli) is an emerging cause of food borne illness. An estimated 73‚000 cases of infection and 61 deaths occur in the United States each year. Infection often leads to bloody diarrhea‚ and occasionally to kidney failure. Most illness has been associated with eating undercooked‚ contaminated ground beef. Person-to-person contact in families and child care centers is also an important mode of transmission. Infection can also occur after drinking raw milk and after swimming
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best indicator of faecal pollution (Edberg et al.‚ 2000). They also indicate the possible presence of pathogenic bacteria‚ viruses and protozoans (Kara et al.‚ 2004). Almost all the methods used to detect total coliforms‚ faecal coliforms and Escherichia coli are enumeration methods that are based on lactose fermentation (American Public Health Association‚ 1992). The Most Probable Number (MPN) method is a statistical‚ multi-step assay consisting of presumptive‚ confirmed and completed phases.
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