Materials 1) Microscope 2) Lab Coat 3) Gram Staining Lab 4) 2 Slides 5) Gram Stain Solution 6) Gram crystal violet 7) Gram iodine 8) Burner 9) Alcohol 10) Water 11) Staphylococcus epidermidis 12) Escherichia coli 13) Bacillus subtillis 14) Gram Safrain 15) Procedures First I get all my materials to get started. I make sure I turn on the burner and sterilize all my materials. I then smear E.C‚ BS‚ & SA and a mixture of all on 2 slides
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Introduction to Microbiology Laboratory report №10 Physical factors affecting growth microbes: Temperature‚ pH and oxygen requirement. Student: Temirlan Aitbekov Lab partner: Kanat Sadykov Instructor: Alessandra Clementi‚ MD‚GP Lab date: 7/11/14 Due date: 14/11/14 Nazarbayev University Abstract: This experiment is directed
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over the course of this experiment: tube inoculation and plate inoculation. We started with plate preparation. Three different lysogeny broth‚ or LB‚ agar plates were prepared for E. coli growth every other week: a control where water was used‚ one for triclosan‚ and one for streptomycin. Using an inoculation loop‚ E. coli was transferred from the test tube to the agar plate. This was done to each plate twice‚ creating a grid-like pattern of bacteria growth. A small paper disk soaked in either water
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Introduction: Escherichia coli (E.coli) is a commensal-pathogenic organism which include a wide range of strains. There are several advanced molecular-genomic technologies that are capable for detecting and identifying different strains of E.coli. But‚ ERIC-PCR technique is a quick and cost effective method for determining individual strains via demonstration of strain specific fingerprint bands. Therefore‚ the ERIC-PCR technique was used to determine the isolated strains of E.coli from different
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Growth and One Step Burst - T7 Phage I. INTRODUCTION: These experiments helped us learn the factors that were involved in the growth of the bacteria that increased our study towards their genetic‚ physical and metabolic characteristics. We used Escherichia coli and Bacteriophage T7 to identify and analyze their identical life cycle and replication that was involved in their process of growth. As‚ growth for any bacteria means it multiplying by increasing in its size and quantity‚ which can easily be
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INTRODUCTION a) Background of the Study Ever since man utilized various species of plants for food‚ clothing‚ as medicines‚ fibers and other useful things. In fact‚ the interaction of man and the plants led to the establishment of the so-called traditional knowledge. One should look into the potential impact of the plant which are generally rich sources of many natural products‚ and its ecological environment and the manner on how it could be utilized for significant use for human welfare
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particularly B. methylotrophicus-SCS2012 exhibited strong antibacterial activity against Streptococcus agalactae‚ Bacillus cereus‚ Escherichia coli‚ Shigella sonnei and Shigella dysenteriae with the zone of inhibition range 28.33 to 32.16 mm at 100μg/disc. Minimum inhibitory concentration (MIC) of the extract against Streptococcus agalactae‚ Bacillus cereus‚ Escherichia coli‚ Shigella sonnei and Shigella dysenteriae was found to be 156‚ 156‚ 312‚ 312 and 625μg/ml respectively.
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Unknown Lab Report Dr. Nathan Cahoone Microbiology 204 December 9‚ 2010 Introduction There are many reasons for knowing the identity of microorganisms. The study and test was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium which I was using unknown #25. Results Unknown #25 had the following morphology on a streak plate: medium sized butyrous cream colored colony. Gram-staining was utilized
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of infected phage particles in the given sample. Method & Materials: * (6) BHI plates at 37˚C * (5) tubes of 9.0mL TSB broth * (6) tubes of soft agar in a 50˚C water bath * T4 phage sample tube * 24hr. broth culture of Escherichia coli B * Sterile pipettes and droppers 1) (Phage Serial-Dilution) a) Label broth tubes with dilution factors (1:10‚ 1:100‚ 1:1000‚ 1:10‚000‚ 1:100‚000). b) Pipet 1.mL of stock to the first dilution. Vortex. c) Pipet
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(Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin). The extracted plasmid DNA is important as it contains ampicillin-resistant gene. As such‚ E. coli cells that have taken up this plasmid DNA will be resistant
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